Team:UTK-Knoxville/31 May 2013
From 2013.igem.org
Team:UK-Knoxville - 2013.igem.org
2013.igem.org
Friday 31 May 13
Digestion of pieces: with the 3 hour method
Backbone (BB) - used the pATBS06 plasmid from before
Enzymes: NdeI and XhoI
Buffer 4
Expected length: 2200bp
There were a few bands on the gel, but I cut out the 2200bp one.
OmpR
Enzymes: NdeI and BamHI
Buffer 3
Expected length: about 720bp
cut out the piece
Aer/EnvZ(piece that was messed up. Just to see if it would actually work)
Enzymes: BglII and XhoI
Buffer 3
Expected length: about 1490bp
Didn’t show up on the gel because the piece was messed up
[Back to Notebook]
-