Team:UTK-Knoxville/5 August 2013


Team:UK-Knoxville -


August 5th 2013

  • Received primers for the TodS sensing domain from Brandon

  • First attempted to PCR amplify the sensing domain from Psuedomonas Putida genomic DNA using a standard PCR protocol and a range of different possible annealing temperatures

  • After all PCR reactions failed to produce any bands (detected via 1% agarose gel), moved to the first method outlined in the following paper.
  • Since these reactions failed as well, August 6, 2013 will be spent using the BSA step PCR method with different organic solvents and in tandem with a touchdown pcr protocol


[back] UTK CBE UTK CBE Trinh Lab