"

From 2013.igem.org

August 5th 2013

• Received primers for the TodS sensing domain from Brandon

• First attempted to PCR amplify the sensing domain from Psuedomonas Putida genomic DNA using a standard PCR protocol and a range of different possible annealing temperatures

• After all PCR reactions failed to produce any bands (detected via 1% agarose gel), moved to the first method outlined in the following paper.
• Since these reactions failed as well, August 6, 2013 will be spent using the BSA step PCR method with different organic solvents and in tandem with a touchdown pcr protocol

 

[back]