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From 2013.igem.org

BioBrick: BBa_E0040

Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0

RFC standard:

RFC 10 BioBrick, nothing was added

ORF from 1 to 714 (excluding stop-codon)

Nucleotide sequence: (underlined part encodes the protein, italic parts were added)  ATGCGTAAA ... CTATACAAATAATAA

Amino acid sequence:   1 MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFF 101 KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY 201 LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Amino acid composition:

A (Ala)

9 (3.8%)

C (Cys)

2 (0.8%)

H (His)

10 (4.2%)

M (Met)

6 (2.5%)

T (Thr)

15 (6.3%)

R (Arg)

7 (2.9%)

Q (Gln)

8 (3.4%)

I (Ile)

12 (5.0%)

F (Phe)

13 (5.5%)

W (Trp)

1 (0.4%)

N (Asn)

13 (5.5%)

E (Glu)

16 (6.7%)

L (Leu)

19 (8.0%)

P (Pro)

10 (4.2%)

Y (Tyr)

11 (4.6%)

D (Asp)

18 (7.6%)

G (Gly)

23 (9.7%)

K (Lys)

20 (8.4%)

S (Ser)

8 (3.4%)

V (Val)

17 (7.1%)

Amino acid counting:

Total number of amino acids (aa):

238

Positively charged aa (Arg + Lys):

27

Negatively charged aa (Asp + Glu):

34

Biochemical parameters:

Molecular weight [Da]:

26909.4

Theoretical pI:

5.8

Extinction coefficient at 280 nm [M-1 cm-1]:

22015 (all Cys as cystine) 21890 (all Cys reduced)

Estimated half-life [h]:

E. coli:

>10

S. cervisiae:

>20

Mammals:

30

Codon usage (CAI):

E. coli:

0.72

S. cervisiae:

0.75

Mammals:

0.68

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.