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Latest revision as of 22:23, 4 September 2013

• Home
• Team
  • Overview
  • Official Team Profile
  • Team Video
  • Undergraduates
  • Mentors
• Project
  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Phage Purification July - August Notebook: Experiments

Overview March-April May-June July-August September-October

7.5 Bacterial Preparation I) Purpose Grow bacteria for later use in propagation of phage. II) Expected Outcome Viable colonies of BL21 and E. coli B. III) Reagants Used E. coli B and BL21 colonies from plates. LB plates (2) IV) Actual Procedure Streak colonies of bacteria in a 4 quadrant pattern and leave in incubator at 37◦ overnight. Parafilm and store in fridge. V) Results Growth of BL21 and E. coli B. The colonies will remain alive for at least one week.