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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period4/Explist
From 2013.igem.org
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<font size="4"> '''8.26 Mutagen Concentration Test - Eighth Protocol''' </font> | <font size="4"> '''8.26 Mutagen Concentration Test - Eighth Protocol''' </font> | ||
| - | + | This is our eighth round of mutagenesis. In some respect, this is the most successful of our mutagenesis yet, because we were able to to get a high titer of phage in each aliquot collected after CsCl purification (with an estimated average of 10E7 pfu/mL). However, because phages are spread out throughout the gradient and did not band, we cannot identify which ones are wild type and which ones are mutant. | |
Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]]. | Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]]. | ||
Revision as of 05:10, 6 September 2013
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8.16 Modeling phage plaque size - Experiment One Add description Detailed procedure: 8.16 Modeling phage plaque size - Experiment One.
8.26 Mutagen Concentration Test - Eighth Protocol This is our eighth round of mutagenesis. In some respect, this is the most successful of our mutagenesis yet, because we were able to to get a high titer of phage in each aliquot collected after CsCl purification (with an estimated average of 10E7 pfu/mL). However, because phages are spread out throughout the gradient and did not band, we cannot identify which ones are wild type and which ones are mutant. Detailed procedure: 8.26 Mutagen Concentration Test - Eighth Protocol.
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