Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period4/Explist

From 2013.igem.org

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: '''Small Phage May - June Notebook: August 17 - August 31 Experiment Listing'''</font>
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: '''Small Phage July - August Notebook: August 17 - August 31 Experiment Listing'''</font>
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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: <u> '''Small Phage''' </u> </font>
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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<font size="4"> '''8.26 Mutagen Concentration Test - Eighth Protocol''' </font>
<font size="4"> '''8.26 Mutagen Concentration Test - Eighth Protocol''' </font>
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This is our eighth round of mutagenesis. In some respect, this is the most successful of our mutagenesis yet, because we were able to to get a high titer of phage in each aliquot collected after CsCl purification (with an estimated average of 10E7 pfu/mL). However, because phages are spread out throughout the gradient and did not band, we cannot identify which ones are wild type and which ones are mutant.
Detailed procedure:  [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]].
Detailed procedure:  [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]].

Latest revision as of 04:28, 9 September 2013


Small Phage July - August Notebook: August 17 - August 31 Experiment Listing



Small Phage
March-April
May-June
July-August
September-October

8.16 Modeling phage plaque size - Experiment One

Add description

Detailed procedure: 8.16 Modeling phage plaque size - Experiment One.


8.26 Mutagen Concentration Test - Eighth Protocol

This is our eighth round of mutagenesis. In some respect, this is the most successful of our mutagenesis yet, because we were able to to get a high titer of phage in each aliquot collected after CsCl purification (with an estimated average of 10E7 pfu/mL). However, because phages are spread out throughout the gradient and did not band, we cannot identify which ones are wild type and which ones are mutant.

Detailed procedure: 8.26 Mutagen Concentration Test - Eighth Protocol.