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Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period3/Explist
From 2013.igem.org
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<font size="4"> '''4.3 Top Agar Stock Preparation''' </font> | <font size="4"> '''4.3 Top Agar Stock Preparation''' </font> | ||
| - | + | This is our recipe for LB and Top Agar. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period3/Exp/4.3 Top Agar Stock Preparation|4.3 Top Agar Stock Preparation]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period3/Exp/4.3 Top Agar Stock Preparation|4.3 Top Agar Stock Preparation]] | ||
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<font size="4"> '''4.5 T7 phage viability assay''' </font> | <font size="4"> '''4.5 T7 phage viability assay''' </font> | ||
| - | + | This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.5 T7 phage viability assay|4.5 T7 phage viability assay]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.5 T7 phage viability assay|4.5 T7 phage viability assay]] | ||
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<font size="4"> '''4.8 T7 phage viability assay 2''' </font> | <font size="4"> '''4.8 T7 phage viability assay 2''' </font> | ||
| - | + | From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+ | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.8 T7 phage viability assay 2|4.8 T7 phage viability assay 2]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.8 T7 phage viability assay 2|4.8 T7 phage viability assay 2]] | ||
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<font size="4"> '''4.10 T7 phage viability assay 3''' </font> | <font size="4"> '''4.10 T7 phage viability assay 3''' </font> | ||
| - | + | We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.10 T7 phage viability assay 3|4.10 T7 phage viability assay 3]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.10 T7 phage viability assay 3|4.10 T7 phage viability assay 3]] | ||
Revision as of 22:57, 5 June 2013
| Small Phage March - April Notebook: April 29 - May 12 Experiment Listing
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4.3 Top Agar Stock Preparation This is our recipe for LB and Top Agar. Detailed procedure: 4.3 Top Agar Stock Preparation
4.5 T7 phage viability assay This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21. Detailed procedure: 4.5 T7 phage viability assay
4.8 T7 phage viability assay 2 From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+ Detailed procedure: 4.8 T7 phage viability assay 2
4.10 T7 phage viability assay 3 We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration. Detailed procedure: 4.10 T7 phage viability assay 3
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