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Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period3/Explist
From 2013.igem.org
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| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook: April | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook: April 1 - April 14 Experiment Listing'''</font> |
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| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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<font size="4"> '''4.3 Top Agar Stock Preparation''' </font> | <font size="4"> '''4.3 Top Agar Stock Preparation''' </font> | ||
| - | + | This is our recipe for LB and Top Agar. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period3/Exp/4.3 Top Agar Stock Preparation|4.3 Top Agar Stock Preparation]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period3/Exp/4.3 Top Agar Stock Preparation|4.3 Top Agar Stock Preparation]] | ||
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| - | <font size="4"> '''4.5 T7 | + | <font size="4"> '''4.5 T7 Phage Viability Assay''' </font> |
| - | + | This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.5 T7 phage viability assay|4.5 T7 phage viability assay]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.5 T7 phage viability assay|4.5 T7 phage viability assay]] | ||
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| - | <font size="4"> '''4.8 T7 | + | <font size="4"> '''4.8 T7 Phage Viability Assay 2''' </font> |
| - | + | From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+ | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.8 T7 phage viability assay 2|4.8 T7 phage viability assay 2]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.8 T7 phage viability assay 2|4.8 T7 phage viability assay 2]] | ||
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| - | <font size="4"> '''4.10 T7 | + | <font size="4"> '''4.10 T7 Phage Viability Assay 3''' </font> |
| - | + | We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration. | |
Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.10 T7 phage viability assay 3|4.10 T7 phage viability assay 3]] | Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.10 T7 phage viability assay 3|4.10 T7 phage viability assay 3]] | ||
Latest revision as of 13:41, 9 September 2013
| Small Phage March - April Notebook: April 1 - April 14 Experiment Listing
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4.3 Top Agar Stock Preparation This is our recipe for LB and Top Agar. Detailed procedure: 4.3 Top Agar Stock Preparation
4.5 T7 Phage Viability Assay This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21. Detailed procedure: 4.5 T7 phage viability assay
4.8 T7 Phage Viability Assay 2 From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+ Detailed procedure: 4.8 T7 phage viability assay 2
4.10 T7 Phage Viability Assay 3 We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration. Detailed procedure: 4.10 T7 phage viability assay 3
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