Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period3/Explist

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<a href="https://2012.igem.org/Team:Cornell/project"><h3 style="margin:0px auto 0px;">Project</h3></a>
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Discover our novel approach to environmental biosensing, where synthetic biology intersects with microbial electrochemical systems.
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook: April 1 - April 14 Experiment Listing'''</font>
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See how our wetlab and drylab subteams operated on a day-to-day basis in order to accomplish our goals of constructing a field-deployable biosensor with direct electric output.
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Meet our dedicated team of undergraduates who conceived of, planned, and carried out all the biological, mechanical and electrical engineering to complete our device.
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Examine our efforts to reach out to our local community &#8212; involving presentations to other scientists, discussions with fellow students and interaction with educators and young children.
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: <u> '''Small Phage''' </u> </font>
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: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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timer : true, // true or false to have the timer
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resetTimerOnClick : false, // true resets the timer instead of pausing slideshow progress
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: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
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<font size="4"> '''4.3 Top Agar Stock Preparation''' </font>
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This is our recipe for LB and Top Agar.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period3/Exp/4.3 Top Agar Stock Preparation|4.3 Top Agar Stock Preparation]]
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<font size="4"> '''4.5 T7 Phage Viability Assay''' </font>
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This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.5 T7 phage viability assay|4.5 T7 phage viability assay]]
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<font size="4"> '''4.8 T7 Phage Viability Assay 2''' </font>
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From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.8 T7 phage viability assay 2|4.8 T7 phage viability assay 2]]
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<font size="4"> '''4.10 T7 Phage Viability Assay 3''' </font>
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We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period2/Exp/4.10 T7 phage viability assay 3|4.10 T7 phage viability assay 3]]
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Latest revision as of 13:41, 9 September 2013


Small Phage March - April Notebook: April 1 - April 14 Experiment Listing



Small Phage
March-April
May-June
July-August
September-October

4.3 Top Agar Stock Preparation

This is our recipe for LB and Top Agar.

Detailed procedure: 4.3 Top Agar Stock Preparation


4.5 T7 Phage Viability Assay

This was used to check if T7 4104 could grow on E. coli BL21 and to see what the concentration of the T7 4104 stock was. However, the T7 4104 could not grow on E. coli BL21.

Detailed procedure: 4.5 T7 phage viability assay


4.8 T7 Phage Viability Assay 2

From the spot tests on E. coli BL21 and E. coli W3110, we determined that T7+ could grow on both and the concentration of T7+

Detailed procedure: 4.8 T7 phage viability assay 2


4.10 T7 Phage Viability Assay 3

We used spot tests of T7+ and T7 (new) to see which phage stock had a greater concentration.

Detailed procedure: 4.10 T7 phage viability assay 3