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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog
From 2013.igem.org
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| - | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: May 27 - June | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: May 27 - June 16 Daily Log'''</font> |
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| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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- Prepared sample for sequencing. This is done as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 T7 Minor Capsid Protein PCR|5.20 T7 Minor Capsid Protein PCR]] | - Prepared sample for sequencing. This is done as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 T7 Minor Capsid Protein PCR|5.20 T7 Minor Capsid Protein PCR]] | ||
| - | - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. | + | - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. |
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| - | <font size="4"> ''' | + | <font size="4"> '''6/10/13''' </font> |
- Made 1250mL of x8 top agar | - Made 1250mL of x8 top agar | ||
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| - | <font size="4"> ''' | + | <font size="4"> '''6/11/13''' </font> |
| - | - | + | - Started approximately 70mL of BL21 liquid culture overnight |
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| - | <font size="4"> ''' | + | <font size="4"> '''6/12/13''' </font> |
| - | - | + | - Perfected protocol for applying mutagen to phage |
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| + | - Started [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]] | ||
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| - | <font size="4"> ''' | + | <font size="4"> '''6/13/13''' </font> |
| - | - | + | - Started approximately 30mL of BL21 liquid culture overnight. |
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| + | <font size="4"> '''6/14/13''' </font> | ||
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| + | - Titered the mutagenesis product from Wednesday as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]] | ||
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| + | <font size="4"> '''6/16/13''' </font> | ||
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| + | - Started approximately 100mL of BL21 liquid culture overnight. | ||
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Latest revision as of 15:30, 9 September 2013
| Small Phage May - June Notebook: May 27 - June 16 Daily Log
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5/28/13 - Started three 8mL E coli BL21 liquid culture at around 4pm.
5/29/13 - Continued 5.20 Mutagen Concentration Experiment - Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes.
5/30/13 - Plates from yesterday are taken out of incubation at around 4:00pm
5/31/13 - Discussed results for 5.20 Mutagen Concentration Experiment -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar. - Discussed plans for next week. - Made new LB and x6 top agar.
6/2/13 - Made about 20ml of BL21 overnight
6/3/13 - Made new LB plates - Plated -4 titer on x6 and x8 plates for 5.20 Mutagen Concentration Experiment
6/5/13 - Discussed plans for the selection process of 5.20 Mutagen Concentration Experiment and calculated the needed volume of agar, overnight, and phage - Made 500mL x8 agar - Performed dilution series to generate enough 200ug -4 phage stock for selection.
6/6/13 - Started approximately 50mL of BL21 liquid culture overnight.
6/7/13 - Performed the selection process of 5.20 Mutagen Concentration Experiment. Specifically, we plated 45 plates of 200ug mutagen, -4 phage dilution using x8 agar. 5 plates of 0ug, -3 phage dilution were also done a control.
6/9/13 - Started 21mL of BL21 overnight.
6/10/13 - Made 1250mL of x8 top agar - Attempted to amplify phage from larger plaques in 5.20 Mutagen Concentration Experiment
6/11/13 - Started approximately 70mL of BL21 liquid culture overnight
6/12/13 - Perfected protocol for applying mutagen to phage - Started 6.12 Mutagen Concentration Test - Second Protocol
6/13/13 - Started approximately 30mL of BL21 liquid culture overnight.
6/14/13 - Titered the mutagenesis product from Wednesday as part of 6.12 Mutagen Concentration Test - Second Protocol
6/16/13 - Started approximately 100mL of BL21 liquid culture overnight.
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