Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period1/Dailylog

Small Phage

March-April

May-June

July-August

September-October

• Performed additional spot tests for 12 samples to verify results of phage concentration in each aliquot for 8.26 Mutagen Concentration Test - Eighth Protocol.

• Started approximately 10mL of E coli B liquid culture overnight.

9/2/13

• Worked on compiling data for 8.26 Mutagen Concentration Test - Eighth Protocol; there are significant differences between CsCl gradient for control sample and that for mutated phage sample.

9/3/13

• Started approximately 20 mL of E coli B liquid culture overnight.

9/4/13

JL, LJP

• Discussed results from recent spot test for 8.26 Mutagen Concentration Test - Eighth Protocol. Phage too spread out to effectively select for mutant phage. Decided to stop until the Phage Purification Team can get the wildtype T7 phage to band.

• Discussed plans and designs for team wiki with Darren and Keltzie.

• Started T7 propagation to generate enough phage for the Phage Purification Team to experiment with. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.

9/5/13

LJP

• Started approximately 10mL of E coli B liquid culture overnight.

9/6/13

JL, LJP

• Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer.

• Took pictures of plates from 8.16 Modeling phage plaque size - Experiment One using alpha imager.

• Divided up assignments for updating notebook.

9/7/13

JL

• Started approximately 15 mL E coli B liquid culture overnight.

• Worked on data analysis / picture processing for 8.16 Modeling phage plaque size - Experiment One.

9/8/13

JL

• Continued data analysis / picture processing for 8.16 Modeling phage plaque size - Experiment One.

• Performed preliminary titer in preparation for repeating the modeling experiment with T1 and T2. For specifics, please see 8.16 Modeling phage plaque size - Experiment One.

• Started approximately 25mL of E coli B liquid culture overnight

9/9/13

JL, LJP

• Discussed analysis of modeling result using ImageJ.

• Repeated the modeling procedure in 8.16 Modeling phage plaque size - Experiment One for T1 and T2. Incubation started at 4:30pm.

• Started a fresh round for phage propagation for the Phage Purification Team. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.

• Divided up assignments for updating the wiki.

8/26/13

- Performed the actual mutagenesis procedure in 8.26 Mutagen Concentration Test - Eighth Protocol.

- Phage Purification Team was able to run the CsCl gradient right after the completion of mutagenesis.

- Started approximately 20mL of E coli B liquid culture overnight.

8/27/13

- Spot test was performed for the post CsCl aliquots (for both control and mutated group) to estimate phage concentration in each. For specific procedure please refer to 8.26 Mutagen Concentration Test - Eighth Protocol.

8/30/13

- Started approximately 20mL of E coli B liquid culture overnight.

- Worked on editing the abstract for submission.

8/31/13

- Further dilution series and spot test was performed to gain a more accurate idea of phage distribution in CsCl gradient. Specific procedures can be found at 8.26 Mutagen Concentration Test - Eighth Protocol.

- Started approximately 10mL of E coli B liquid culture overnight.