Team:UNITN-Trento/Parts

From 2013.igem.org

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Revision as of 07:31, 20 September 2013

Ethylene

☺ ☺ ☺	BBa_K1065000	EFE
	Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

☺ ☺ ☺	BBa_K1065002	EFE with double-terminator
	EFE followed by the double terminator BBa_B0015.

☺ ☺ ☺	BBa_K1065001	EFE arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

Methyl salicylate

☺ ☺ ☺	BBa_K1065100	BSMT1
	This part encodes the BSMT1, it is the enzyme that methylates salycilic acid to obtain methyl salycilate. It’s the original part from the Eau de coli MIT iGEM 2006 project, resubmitted by us in pSB1C3.

☺ ☺ ☺	BBa_K1065103	PchBA
	PchA ,the enzyme catalyzing salicylate biosynthesis isochorismate, and PchB that is the isochorismate-pyruvate lyase for that form the precursor for the salycilic acid.

☺ ☺ ☺	BBa_K1065104	SAM synthetase
	This part encodes the SAM (S-AdoMet) synthetase gene, (which we friendly called "uncle SAM") . It is a methyl donor required to produce MeSA and other methylated molecules.

☺ ☺ ☺	BBa_K1065101	BSMT1 arabinose-regulated
	A methyltransferase (BSMT1) regulated by the araCpBAD inducible promoter.

☺ ☺ ☺	Bba_K1065105	SAM synthetase IPTG-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

☺ ☺ ☺	BBa_K1065102	PchBA IPTG-regulated and BSMT1 arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

☺ ☺ ☺	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

Blue light

☺ ☺ ☺	Bba_K1065301	amilCP IPTG-regulated
	We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party!

☺ ☺ ☺	Bba_K1065300	BBa_K952003 with Yf1 and FixJ IPTG-regulated.
	pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.

☺ ☺ ☺	a[href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303]{BBa_K1065303}	Yf1+FixJ+pFixK2
	The starting point to build a blue-light sensing circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006.

☺ ☺ ☺	BBa_K1065310	Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP
	This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

☺ ☺ ☺	a[href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302]{BBa_K1065302}	BBa_K1065300 improved
	The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP.

☺ ☺ ☺	BBa_K1065305	BBa_K952003 improved
	The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP.

☺ ☺ ☺	BBa_K1065306	J23100+Yf1+FixJ
	Yf1 and FixJ under the Anderson promoter (BBa_J23100).

☺ ☺ ☺	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

☺ ☺ ☺	BBa_K1065309	BBa_K1065302+BBa_K1065002
	Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.

Bacillus

☺ ☺ ☺	BBa_K1065201	pXyl+GFP
	An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

☺ ☺ ☺	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

☺ ☺ ☺	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

☺ ☺ ☺	BBa_K1065311	Complete blue-light sensing device with inverter, amilCP and EFE
	Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.

[http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Parts&action=edit Edit this page] | Main Page

@@ Line 23: / Line 23: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065000</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065000">BBa_K1065000</a></td>
                          <td class="name">EFE</td>
                      </tr>
@@ Line 38: / Line 38: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065002</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065002">BBa_K1065002</a></td>
                          <td class="name">EFE with double-terminator</td>
                      </tr>
@@ Line 53: / Line 53: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065001</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065001">BBa_K1065001</a></td>
                          <td class="name">EFE arabinose-regulated</td>
                      </tr>
@@ Line 75: / Line 75: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065100</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065100">BBa_K1065100</a></td>
                          <td class="name">BSMT1</td>
                      </tr>
@@ Line 90: / Line 90: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065103</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065103">BBa_K1065103</a></td>
                          <td class="name">PchBA</td>
                      </tr>
@@ Line 105: / Line 105: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065104</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065104">BBa_K1065104</a></td>
                          <td class="name">SAM synthetase</td>
                      </tr>
@@ Line 120: / Line 120: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065101</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065101">BBa_K1065101</a></td>
                          <td class="name">BSMT1 arabinose-regulated</td>
                      </tr>
@@ Line 135: / Line 135: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">Bba_K1065105</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065105">Bba_K1065105</a></td>
                          <td class="name">SAM synthetase IPTG-regulated</td>
                      </tr>
@@ Line 150: / Line 150: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065102</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065102">BBa_K1065102</a></td>
                          <td class="name">PchBA IPTG-regulated and BSMT1 arabinose-regulated</td>
                      </tr>
@@ Line 165: / Line 165: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065106</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td>
                          <td class="name">BSMT1, PchBA and SAM synthetase</td>
                      </tr>
@@ Line 217: / Line 217: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065303</td>
+                         <td class="code">a[href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303]{BBa_K1065303}</td>
                          <td class="name">Yf1+FixJ+pFixK2</td>
                      </tr>
@@ Line 232: / Line 232: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065310</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td>
                          <td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td>
                      </tr>
@@ Line 247: / Line 247: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065302</td>
+                         <td class="code">a[href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302]{BBa_K1065302}</td>
                          <td class="name">BBa_K1065300 improved</td>
                      </tr>
@@ Line 262: / Line 262: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065305</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td>
                          <td class="name">BBa_K952003 improved</td>
                      </tr>
@@ Line 277: / Line 277: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065306</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td>
                          <td class="name">J23100+Yf1+FixJ</td>
                      </tr>
@@ Line 292: / Line 292: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065204</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
                          <td class="name">pXyl+EFE</td>
                      </tr>
@@ Line 307: / Line 307: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065309</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065309">BBa_K1065309</a></td>
                          <td class="name">BBa_K1065302+BBa_K1065002</td>
                      </tr>
@@ Line 329: / Line 329: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065201</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065201">BBa_K1065201</a></td>
                          <td class="name">pXyl+GFP</td>
                      </tr>
@@ Line 344: / Line 344: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065106</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td>
                          <td class="name">BSMT1, PchBA and SAM synthetase</td>
                      </tr>
@@ Line 359: / Line 359: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065204</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td>
                          <td class="name">pXyl+EFE</td>
                      </tr>
@@ Line 374: / Line 374: @@
                      <tr>
                          <td rowspan="2" class="grade">☺ ☺ ☺</td>
-                         <td class="code">BBa_K1065311</td>
+                         <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td>
                          <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td>
                      </tr>