19/09/13

From 2013.igem.org

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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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|}
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==DNA isolation from PCR products==
==DNA isolation from PCR products==
*Isolating DNA using Omega nucleic acid purification kit and following its protocol.
*Isolating DNA using Omega nucleic acid purification kit and following its protocol.
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|Sample nr||Volume ul||Concentration ng/ul||260/280||260/230
|Sample nr||Volume ul||Concentration ng/ul||260/280||260/230
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|-
-
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|X3||41||11.3||1.87||1.29
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|-
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|X5||47||12.2||1.78||1.22
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|-
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|X6||34||12.9||1.79||1.22
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|-
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|X7||45||8||1.71||0.96
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|-
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|X8||43||10.5||1.88||1.2
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|-
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|X10||44||9||1.88||1.2
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|-
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|X12||45||12.8||1.70||1.40
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|-
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|F1||44.5||11.2||1.69||1.22
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|-
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|F2||44||9.1||1.88||1.20
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|-
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|F3||45.2||10.6||1.77||0.99
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|-
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|F4||43.7||9.7||1.82||1.10
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|-
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|F5||45.2||10.6||1.67||1.19
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|-
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|F6||45.4||9.6||1.82||0.85
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|-
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|F7||46||11||1.76||1.14
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|-
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|F8||46||11.7||1.73||1.24
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|F9||42.4||10.5||1.83||1.32
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|-
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|F10||44.3||11||1.93||1.39
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|-
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|F11||45.5||10.3||1.82||1.18
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|-
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|F12||45.5||14.9||1.83||1.32
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|-
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|B1||45||6.9||1.56||0.86
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|-
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|B2||46||10.5||1.64||1.17
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|-
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|B3||46||10||1.78||1.41
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|-
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|B4||47||11.5||1.70||1.35
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|-
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|B5||47||10.4||1.84||1.25
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|B6||47||8.6||1.96||1.33
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|-
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|B7||47||9.7||1.74||1.01
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|-
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|B8||46||10.7||1.86||1.22
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|-
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|B9||45||12.4||1.58||1.05
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|-
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|B10||46||11.1||1.73||1.32
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|-
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|B11||46||10.2||1.87||1.56
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|-
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|B12||46||12.1||1.75||1.38
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|}
==Sending isolated DNA to be sequenced by PNACL==
==Sending isolated DNA to be sequenced by PNACL==

Latest revision as of 10:49, 20 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Contents

DNA isolation from PCR products

  • Isolating DNA using Omega nucleic acid purification kit and following its protocol.
  • Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
  • Selection was made by looking at gel pictures and selecting samples with visible bands.

Nanodropping the samples for concentration

Sample nrVolume ulConcentration ng/ul260/280260/230
X34111.31.871.29
X54712.21.781.22
X63412.91.791.22
X74581.710.96
X84310.51.881.2
X104491.881.2
X124512.81.701.40
F144.511.21.691.22
F2449.11.881.20
F345.210.61.770.99
F443.79.71.821.10
F545.210.61.671.19
F645.49.61.820.85
F746111.761.14
F84611.71.731.24
F942.410.51.831.32
F1044.3111.931.39
F1145.510.31.821.18
F1245.514.91.831.32
B1456.91.560.86
B24610.51.641.17
B346101.781.41
B44711.51.701.35
B54710.41.841.25
B6478.61.961.33
B7479.71.741.01
B84610.71.861.22
B94512.41.581.05
B104611.11.731.32
B114610.21.871.56
B124612.11.751.38

Sending isolated DNA to be sequenced by PNACL

  • Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

Preparing overnight broths

  • Preparing broths from individual colonie plates from previous day
  • Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
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