"
Team:UNITN-Trento/Parts
From 2013.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065000">BBa_K1065000</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065000">BBa_K1065000</a></td> | ||
<td class="name">EFE</td> | <td class="name">EFE</td> | ||
| Line 38: | Line 41: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065002">BBa_K1065002</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065002">BBa_K1065002</a></td> | ||
<td class="name">EFE with double-terminator</td> | <td class="name">EFE with double-terminator</td> | ||
| Line 53: | Line 59: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065001">BBa_K1065001</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065001">BBa_K1065001</a></td> | ||
<td class="name">EFE arabinose-regulated</td> | <td class="name">EFE arabinose-regulated</td> | ||
| Line 75: | Line 85: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065100">BBa_K1065100</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065100">BBa_K1065100</a></td> | ||
<td class="name">BSMT1</td> | <td class="name">BSMT1</td> | ||
| Line 90: | Line 103: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065103">BBa_K1065103</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065103">BBa_K1065103</a></td> | ||
<td class="name">PchBA</td> | <td class="name">PchBA</td> | ||
| Line 105: | Line 121: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065104">BBa_K1065104</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065104">BBa_K1065104</a></td> | ||
<td class="name">SAM synthetase</td> | <td class="name">SAM synthetase</td> | ||
| Line 120: | Line 139: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065101">BBa_K1065101</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065101">BBa_K1065101</a></td> | ||
<td class="name">BSMT1 arabinose-regulated</td> | <td class="name">BSMT1 arabinose-regulated</td> | ||
| Line 135: | Line 158: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065105">Bba_K1065105</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065105">Bba_K1065105</a></td> | ||
<td class="name">SAM synthetase under pLac promoter</td> | <td class="name">SAM synthetase under pLac promoter</td> | ||
| Line 150: | Line 177: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065102">BBa_K1065102</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065102">BBa_K1065102</a></td> | ||
<td class="name">PchBA IPTG-regulated and BSMT1 arabinose-regulated</td> | <td class="name">PchBA IPTG-regulated and BSMT1 arabinose-regulated</td> | ||
| Line 165: | Line 196: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065106">BBa_K1065106</a></td> | ||
<td class="name">BSMT1, PchBA and SAM synthetase</td> | <td class="name">BSMT1, PchBA and SAM synthetase</td> | ||
| Line 187: | Line 222: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code">Bba_K1065301</td> | <td class="code">Bba_K1065301</td> | ||
<td class="name">amilCP IPTG-regulated</td> | <td class="name">amilCP IPTG-regulated</td> | ||
| Line 202: | Line 241: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code">Bba_K1065300</td> | <td class="code">Bba_K1065300</td> | ||
<td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td> | <td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td> | ||
| Line 217: | Line 260: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td> | ||
<td class="name">Yf1+FixJ+pFixK2</td> | <td class="name">Yf1+FixJ+pFixK2</td> | ||
| Line 232: | Line 279: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> | ||
<td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td> | <td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td> | ||
| Line 247: | Line 298: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td> | ||
<td class="name">BBa_K1065300 improved</td> | <td class="name">BBa_K1065300 improved</td> | ||
| Line 262: | Line 317: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td> | ||
<td class="name">BBa_K952003 improved</td> | <td class="name">BBa_K952003 improved</td> | ||
| Line 277: | Line 336: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td> | ||
<td class="name">J23100+Yf1+FixJ</td> | <td class="name">J23100+Yf1+FixJ</td> | ||
| Line 292: | Line 355: | ||
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td rowspan="2" class="grade"><img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | + | <td rowspan="2" class="grade"> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | </td> | ||
<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065204">BBa_K1065204</a></td> | ||
<td class="name">pXyl+EFE</td> | <td class="name">pXyl+EFE</td> | ||
Revision as of 21:29, 20 September 2013
|
BBa_K1065000 | EFE |
| Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. | ||
|
|
BBa_K1065002 | EFE with double-terminator |
| EFE followed by the double terminator BBa_B0015. | ||
|
|
BBa_K1065001 | EFE arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
|
|
BBa_K1065100 | BSMT1 |
| This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3. | ||
|
|
BBa_K1065103 | PchBA |
| PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. | ||
|
|
BBa_K1065104 | SAM synthetase |
| This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules. | ||
|
|
BBa_K1065101 | BSMT1 arabinose-regulated |
| inducible A methyltransferase (BSMT1) regulated by the araCpBAD promoter. | ||
|
|
Bba_K1065105 | SAM synthetase under pLac promoter |
| This is SAM synthetase (uncle SAM) regulated by the costitutive Lac promoter. | ||
|
|
BBa_K1065102 | PchBA IPTG-regulated and BSMT1 arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
|
|
BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production. | ||
|
|
Bba_K1065301 | amilCP IPTG-regulated |
| We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party! | ||
|
|
Bba_K1065300 | BBa_K952003 with Yf1 and FixJ IPTG-regulated. |
| pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream. | ||
|
|
BBa_K1065303 | Yf1+FixJ+pFixK2 |
| The starting point to build a blue-light sensing circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006. | ||
|
|
BBa_K1065310 | Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP |
| This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | ||
|
|
BBa_K1065302 | BBa_K1065300 improved |
| The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP. | ||
|
|
BBa_K1065305 | BBa_K952003 improved |
| The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP. | ||
|
|
BBa_K1065306 | J23100+Yf1+FixJ |
| Yf1 and FixJ under the Anderson promoter (BBa_J23100). | ||
|
|
BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
| |
BBa_K1065309 | BBa_K1065302+BBa_K1065002 |
| Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | ||
| |
BBa_K1065201 | pXyl+GFP |
| An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production. | ||
| |
BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | ||
| |
BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
| |
BBa_K1065311 | Complete blue-light sensing device with inverter, amilCP and EFE |
| Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | ||
- Login
- [http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Parts&action=edit Edit this page]
- Upload File
- Sitemap
[http://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Parts&action=edit Edit this page] | Main Page
