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Team:Nanjing-China/consideration
From 2013.igem.org
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<li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#safety">Safety</a></li> | <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#safety">Safety</a></li> | ||
<li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#gene">Gene Guard</a></li> | <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#gene">Gene Guard</a></li> | ||
| + | <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#Industrialization">Industrialization</a></li> | ||
| + | <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/consideration#Achievement">Achievement</a></li> | ||
</ul> | </ul> | ||
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As our project suggests the release of genetically modified organisms (GMOs) into the environment, we feel it is necessary to reduce the risk of horizontal gene transfer. To prevent horizontal gene transfer of the genes, we have designed a system based on the genes encoding holin/endolysin and anti-holin, Colicin-E3 and Colicin Immunity E3, Endonuclease EcoRI and Methyltransferase EcoRI. There is no doubt that our multi-containment systemis more secure than a single-containment system.<br/><br/> | As our project suggests the release of genetically modified organisms (GMOs) into the environment, we feel it is necessary to reduce the risk of horizontal gene transfer. To prevent horizontal gene transfer of the genes, we have designed a system based on the genes encoding holin/endolysin and anti-holin, Colicin-E3 and Colicin Immunity E3, Endonuclease EcoRI and Methyltransferase EcoRI. There is no doubt that our multi-containment systemis more secure than a single-containment system.<br/><br/> | ||
Holin/Endolysin will be carried on a plasmid and the anti-holin on the genomic DNA. The other gene pairs act in the same the way (Fig. 7-2-1). Anti-holin will prevent the holin and endolysin encoded on the plasmids to lyse the cell. However, if the plasmid is transferred to any other bacterial that is not our modified bacteria, it will induce cell lysis, thereby preventing the spread of genetic information contained in our genetically modified bacteria. And the other gene pairs act in the same the way. Therefore, we can prevent the transfer of synthetic genes into wild type bacteria by our treble safe guard. | Holin/Endolysin will be carried on a plasmid and the anti-holin on the genomic DNA. The other gene pairs act in the same the way (Fig. 7-2-1). Anti-holin will prevent the holin and endolysin encoded on the plasmids to lyse the cell. However, if the plasmid is transferred to any other bacterial that is not our modified bacteria, it will induce cell lysis, thereby preventing the spread of genetic information contained in our genetically modified bacteria. And the other gene pairs act in the same the way. Therefore, we can prevent the transfer of synthetic genes into wild type bacteria by our treble safe guard. | ||
| + | </dd> | ||
| + | </dl> | ||
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| + | <dt><a name="Industrialization">Industrialization</a></dt> | ||
| + | <dd class="dd_1"> | ||
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| + | </dd> | ||
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| + | <dt><a name="Achievement">Achievement</a></dt> | ||
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</dl> | </dl> | ||
Revision as of 03:22, 24 September 2013
- Safety
- Q1: If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, whatrisks might arise? Also, whatrisks might arise if the knowledge you generate or the methods you develop became widely available?
A: The strain we use is K12, which is not associated with diseases in healthy adult humans. However, if largely produced, they might affect the living situations of other creatures. For example, they might cause living competition on account of limited living resources. As to the second question, the methods we use in our project are quite common in Molecular Biology experiment, there will not be special risks arising.
Q2: Does your project include any design features to address safety risks?
A: Yes, we have a system to make sure that the system we construct can only take effect and be inherited within our own strain. In other words, our system won't affect other living creatures in the environment.
Q3: Do your team members perform experiments under the guidelines of the Laboratory Biosafety Manual?
A: Yes, all of our member had read the manual carefully before we started to perform our experiments. Things like putting on gloves and wearing lab-gowns are strictly implemented in our lab. What's more, the atrazine solution which we left were all collected and treated with appropriated chemical solutions before they are released into the environment. And all the bacteria-related instruments were all sterilized before we threw them out.
- Gene Guard
-
As our project suggests the release of genetically modified organisms (GMOs) into the environment, we feel it is necessary to reduce the risk of horizontal gene transfer. To prevent horizontal gene transfer of the genes, we have designed a system based on the genes encoding holin/endolysin and anti-holin, Colicin-E3 and Colicin Immunity E3, Endonuclease EcoRI and Methyltransferase EcoRI. There is no doubt that our multi-containment systemis more secure than a single-containment system.
Holin/Endolysin will be carried on a plasmid and the anti-holin on the genomic DNA. The other gene pairs act in the same the way (Fig. 7-2-1). Anti-holin will prevent the holin and endolysin encoded on the plasmids to lyse the cell. However, if the plasmid is transferred to any other bacterial that is not our modified bacteria, it will induce cell lysis, thereby preventing the spread of genetic information contained in our genetically modified bacteria. And the other gene pairs act in the same the way. Therefore, we can prevent the transfer of synthetic genes into wild type bacteria by our treble safe guard.
