Team:Tokyo Tech
From 2013.igem.org
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Revision as of 10:43, 24 September 2013
Abstract
We intend to send a message to society so as to let them know the development of synthetic biology, especially the development of the network programming, as well as we enjoy our activity for iGEM. |
[[File:tokyotechprojectoverview.png|250px|right|[Fig1. Human Practice experiment] We did experiment class for high school students. ]]
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[Fig2. Human Practice questionnaire]
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Ninja is a Japan’s ancient spy-warrior. Usually Ninja disguises himself as an ordinary civilian in public. Once he detects samurai who is the assassination target, he immediately gets ready for battle. He defeats samurai with shuriken, throwing knives. |
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In our programing of artificial genetic circuit, E. ninja heads the cast. In response to E. civilian signal or E. samurai signal, E. ninja changes its state: “mimic state” and “attack state”. The circuit of E. ninja contains a bi-stable switch and a signal dependent switching part. We decided to use C6-AHL and C12-AHL as signals. The crosstalk between the two signals in synthetic biology, is well known as a big problem to be addressed. To realize accurate switching, by network engineering, we have succeeded in circumvention of the crosstalk that occurs in bacterial cell-cell communication system. (fig.3) (fig4.) In addition, E. ninja releases M13 phage, which corresponds to shuriken, when it receives E. samurai signal. The inducible phage release will open new synthetic biology by programmed DNA messaging. (fig.5) |
[Fig3. Crosstalk assay] |
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In the second-life story, E. ninja starts farming in a peaceful village. It can synthesize plant hormone efficiently, depending on the soil environment. We constructed a new phosphate sensor (phoA promoter). Also, we learned methods for quantitative analysis for activity of cytokinin, a plant hormone, through a bioassay of cucumber seed sprouts. Towards further consideration of farming with microbes, we have also continued the human practice investigation through some interviews with Science Foundation or Organizations spreading the science. (fig.6) |
[Fig5. Bioassay of plant]
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