"
Team:Newcastle
From 2013.igem.org
| Line 109: | Line 109: | ||
</div> | </div> | ||
<div class="under_text"> | <div class="under_text"> | ||
| + | <a href="https://2013.igem.org/Team:Newcastle/Notebook/achievements"> | ||
<img src="https://static.igem.org/mediawiki/2013/b/b6/BareCillus_Achievements_txt.png" /> | <img src="https://static.igem.org/mediawiki/2013/b/b6/BareCillus_Achievements_txt.png" /> | ||
</div> | </div> | ||
| Line 117: | Line 118: | ||
</div> | </div> | ||
<div class="under_text"> | <div class="under_text"> | ||
| + | <a href="https://2013.igem.org/Team:Newcastle/Outreach/Overview"> | ||
<img src="https://static.igem.org/mediawiki/2013/7/75/BareCillus_Outreach_txt.png" /> | <img src="https://static.igem.org/mediawiki/2013/7/75/BareCillus_Outreach_txt.png" /> | ||
</div> | </div> | ||
| Line 125: | Line 127: | ||
</div> | </div> | ||
<div class="under_text"> | <div class="under_text"> | ||
| + | <a href="https://2013.igem.org/Team:Newcastle/Collaborations"> | ||
<img src="https://static.igem.org/mediawiki/2013/c/c1/BareCillus_Collab_txt.png" /> | <img src="https://static.igem.org/mediawiki/2013/c/c1/BareCillus_Collab_txt.png" /> | ||
</div> | </div> | ||
Revision as of 16:33, 24 September 2013
Our Project
Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose L-forms as a novel chassis for synthetic biology. Our principle BioBrick switches Bacillus subtilis cells between rod-shape and L-form.
We will use microfluidics to attempt genome shuffling and shape-shifting. It is easier to fuse bacteria without cell walls. Fusion will cause genetic recombination, allowing directed evolution. We will put L-forms in moulds to observe if they adopt different shapes.
L-forms exist symbiotically in plants, which we will visualise by growing GFP labelled L-forms inside seedlings. L-forms could be engineered to supply nutrients to their host. L-forms are osmotically sensitive, giving biosecurity that they lyse if they escape from the plant.
As outreach we reflected upon our project's implications with stakeholders, created a BioGame for the public and developed a workshop for those new to modelling. Finally, we evaluated the relationship between synthetic biology and architecture.
