Team:USTC CHINA/Notebook/Protocols/Gel Extraction
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Add 0.5 × Buffer DE-A volume of Buffer DE-B.</br> | Add 0.5 × Buffer DE-A volume of Buffer DE-B.</br> | ||
- | 3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min)</br> | + | 3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).</br> |
- | 4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min) | + | 4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).</br> |
- | Repeat wash with Buffer W2</br> | + | Repeat wash with Buffer W2.</br> |
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.</br> | Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.</br> | ||
- | 5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min)</br> | + | 5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).</br> |
Revision as of 16:11, 25 September 2013