Team:NJU China/Project/Brain

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<html lang="en">
+
<!DOCTYPE html>
-
    <head>
+
<html lang="en" class="no-js">
-
        <meta charset="UTF-8" />
+
<head>
-
        <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">  
+
<meta charset="UTF-8" />
-
         <meta name="viewport" content="width=device-width, initial-scale=1.0">  
+
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">  
-
<style type="text/css">
+
         <meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
<title>NJU_China</title>
 +
<meta name="description" content="Circular Navigation Styles - Building a Circular Navigation with CSS Transforms | Codrops " />
 +
<meta name="keywords" content="css transforms, circular navigation, round navigation, circular menu, tutorial" />
 +
<meta name="author" content="Sara Soueidan for Codrops" />
 +
<link rel="shortcut icon" href="../favicon.ico">
 +
<script src="http://cdn.jquerytools.org/1.2.5/full/jquery.tools.min.js?foo"></script>
 +
 
 +
<link rel="stylesheet" type="text/css" href="http://cs.wellesley.edu/~hcilab/iGEM2012/css/Team.css">
 +
<STYLE type="text/css">
/* hiding the top section*/
/* hiding the top section*/
body{position:absolute; top:0px; width:100%; height:1480px;}
body{position:absolute; top:0px; width:100%; height:1480px;}
Line 59: Line 69:
     float:left;
     float:left;
     white-space: nowrap;
     white-space: nowrap;
-
     top:-3px;
+
     top:-6px;
     width: 490px;
     width: 490px;
     z-index: 5000;
     z-index: 5000;
     font-family: sans-serif;
     font-family: sans-serif;
-
     font-size: 55%;
+
     font-size: 95%;
     line-height: 1em;
     line-height: 1em;
     z-index:99;
     z-index:99;
Line 131: Line 141:
/* end menu (edit, page ...) */
/* end menu (edit, page ...) */
-
/* CSS reset */
+
 
-
body,div,dl,dt,dd,ul,ol,li,h1,h2,h3,h4,h5,h6,pre,form,fieldset,input,textarea,p,blockquote,th,td {  
+
/* HEADER STYLES: banner, navbar, etc. */
-
margin:0;
+
 
-
padding:0;
+
ul#nav {
 +
width:1200px;
 +
margin:0 0 0 30px;
 +
position:relative;
}
}
-
html,body {
+
 
 +
#nav li {
 +
 
 +
color: #FFFFFF;
 +
background-color:none;
 +
margin: 0 50px 0 30px;
 +
float: left;
 +
position: relative;
 +
list-style: none;
 +
text-transform:uppercase;
 +
}
 +
#nav li:last-child { margin:0; }
 +
 
 +
 
 +
/* dropdown */
 +
#nav li:hover > ul {
 +
/*display: block;*/
 +
opacity:1;
margin:0;
margin:0;
-
padding:0;
+
background-color: none;
 +
z-index:0;
}
}
-
table {
+
 
-
border-collapse:collapse;
+
/* level 2 list */
-
border-spacing:0;
+
#nav ul {
 +
/*display: none;*/
 +
opacity:0;
 +
margin: 200px 0 0 0;
 +
padding: 70px 0 0 0;
 +
width: 205px;
 +
position: absolute;
 +
left: 0;
 +
z-index:-1;
 +
-webkit-transition-duration:0.5s;
 +
-moz-transition-duration:0.5s;
 +
-o-transition-duration:0.5s;
}
}
-
fieldset,img {  
+
#nav ul li {
-
border:0;
+
float: none;
 +
margin: 0;
 +
padding: 0;
}
}
-
input{
+
 
-
border:1px solid #b0b0b0;
+
#nav ul a {
-
padding:3px 5px 4px;
+
font-weight: normal;
-
color:#979797;
+
/*text-shadow: 0 1px 0 #fff;*/
-
width:190px;
+
}
}
-
address,caption,cite,code,dfn,th,var {
+
 
-
font-style:normal;
+
 
-
font-weight:normal;
+
</STYLE>
 +
 
 +
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:400,600' rel='stylesheet' type='text/css'>
 +
 
 +
 
 +
<style type="text/css">
 +
@import url(http://fonts.googleapis.com/css?family=Lato:300,400,700);
 +
@font-face {
 +
font-family: 'codropsicons';
 +
src:url('../fonts/codropsicons/codropsicons.eot');
 +
src:url('../fonts/codropsicons/codropsicons.eot?#iefix') format('embedded-opentype'),
 +
url('../fonts/codropsicons/codropsicons.woff') format('woff'),
 +
url('../fonts/codropsicons/codropsicons.ttf') format('truetype'),
 +
url('../fonts/codropsicons/codropsicons.svg#codropsicons') format('svg');
 +
font-weight: normal;
 +
font-style: normal;
}
}
-
ol,ul {
+
 
-
list-style:none;
+
.container > header,
 +
.codrops-top,
 +
.container > section {
 +
font-family: 'Lato', Arial, sans-serif;
}
}
-
caption,th {
+
 
-
text-align:left;
+
.container > header {
 +
margin: 0 auto;
 +
padding: 2em;
 +
text-align: center;
 +
background: rgba(0,0,0,0.01);
}
}
-
h1,h2,h3,h4,h5,h6 {
+
 
-
font-size:100%;
+
.container > header h1 {
-
font-weight:normal;
+
font-size: 2.625em;
 +
line-height: 1.3;
 +
margin: 0;
 +
font-weight: 300;
}
}
-
q:before,q:after {
+
 
-
content:'';
+
.container > header span {
 +
display: block;
 +
font-size: 60%;
 +
opacity: 0.7;
 +
padding: 0 0 0.6em 0.1em;
}
}
-
abbr,acronym { border:0;
+
 
 +
/* To Navigation Style */
 +
.codrops-top {
 +
background: #f06060;
 +
background: rgba(255, 255, 255, 0.1);
 +
text-transform: uppercase;
 +
width: 100%;
 +
font-size: 0.69em;
 +
line-height: 2.2;
}
}
-
/* General Demo Style */
+
.codrops-top a {
-
body{
+
text-decoration: none;
-
    background: #f7f7f7 url(../images/old_wall.jpg) repeat top left;
+
padding: 0 1em;
-
    color: rgba(0,0,0,0.8);
+
letter-spacing: 0.1em;
-
    font-family: 'Kelly Slab','Myriad Pro', Arial, sans-serif;
+
color: #fff;
-
    font-size: 15px;
+
display: inline-block;
}
}
-
a{
+
 
-
    color: #fff;
+
.codrops-top a:hover {
-
    text-decoration: none;
+
background: rgba(255,255,255,0.95);
 +
color: #777;
}
}
-
.container{
+
 
-
    width: 100%;
+
.codrops-top span.right {
-
    position: relative;
+
float: right;
}
}
-
.clr{
+
 
-
    clear: both;
+
.codrops-top span.right a {
 +
float: left;
 +
display: block;
}
}
-
h1{
+
 
-
    font-size: 24px;
+
.codrops-icon:before {
-
    position:relative;
+
font-family: 'codropsicons';
-
    font-weight: 400;
+
margin: 0 4px;
-
    padding: 30px 5px 10px 5px;
+
speak: none;
-
margin: 0px 15px;
+
font-style: normal;
-
color: rgba(153,157,181,0.6);
+
font-weight: normal;
-
text-shadow: 0px 1px 1px #fff;
+
font-variant: normal;
-
border-bottom: 1px solid rgba(17,17,22,0.1);
+
text-transform: none;
 +
line-height: 1;
 +
-webkit-font-smoothing: antialiased;
}
}
-
/* Header Style */
+
 
-
.header{
+
.codrops-icon-drop:before {
-
    font-family:'Arial Narrow', Arial, sans-serif;
+
content: "\e001";
-
    line-height: 24px;
+
-
    font-size: 11px;
+
-
    background: #000;
+
-
    opacity: 0.9;
+
-
    text-transform: uppercase;
+
-
    z-index: 9999;
+
-
    position: relative;
+
-
    -moz-box-shadow: 1px 0px 2px #000;
+
-
    -webkit-box-shadow: 1px 0px 2px #000;
+
-
    box-shadow: 1px 0px 2px #000;
+
}
}
-
.header a{
+
 
-
    padding: 0px 10px;
+
.codrops-icon-prev:before {
-
    letter-spacing: 1px;
+
content: "\e004";
-
    color: #ddd;
+
-
    display: block;
+
-
    float: left;
+
}
}
-
.header a:hover{
+
 
-
    color: #fff;
+
/* Demo Buttons Style */
 +
.codrops-demos {
 +
padding-top: 1em;
 +
font-size: 0.9em;
}
}
-
.header span.right{
+
 
-
    float: right;
+
.codrops-demos a {
 +
text-decoration: none;
 +
outline: none;
 +
display: inline-block;
 +
margin: 0.5em;
 +
padding: 0.7em 1.1em;
 +
border: 3px solid #fff;
 +
color: #fff;
 +
font-weight: 700;
}
}
-
.header span.right a{
+
 
-
    float: none;
+
.codrops-demos a:hover,
-
    display: inline;
+
.codrops-demos a.current-demo,
 +
.codrops-demos a.current-demo:hover {
 +
opacity: 0.6;
}
}
-
.demos{
+
 
-
    position:relative;
+
.container > section {
-
    z-index: 1000;
+
padding: 2em;
-
    text-align:center;
+
font-size: 1.4em;
-
float: right;
+
max-width: 50em;
-
padding: 10px;
+
margin: 0 auto;
}
}
-
.demos a{
+
 
-
    display: inline-block;
+
.container > section p {
-
    padding: 4px 6px;
+
padding: 0.8em 0;
-
    background: #000;
+
text-align: justify;
-
    color: #fff;
+
opacity: 0.5;
-
    text-transform: uppercase;
+
font-weight: 300;
-
font-family: Arial;
+
line-height: 1.4;
-
    font-size: 11px;
+
-
-webkit-font-smoothing:antialiased;
+
-
    box-shadow: 1px 1px 4px rgba(0,0,0,0.2);
+
-
    -webkit-transition: all 0.2s linear;
+
-
    -moz-transition: all 0.2s linear;
+
-
    -o-transition: all 0.2s linear;
+
-
    -ms-transition: all 0.2s linear;
+
-
    transition: all 0.2s linear;
+
}
}
-
.demos a:hover{
+
 
-
    color: #fff;
+
@media screen and (max-width: 25em) {
-
    background: #c90047;
+
 
-
}
+
.codrops-icon span {
-
.demos a.current-demo,
+
display: none;
-
.demos a.current-demo:hover{
+
}
-
    font-weight: bold;
+
 
-
    background: #c90047;
+
-
    color: #fff;
+
-
cursor: default;
+
}
}
</style>
</style>
<style type="text/css">
<style type="text/css">
-
h2.ss-subtitle{
+
* {
-
    padding: 10px 10px 40px;
+
-
    font-size: 52px;
+
-
    text-transform: uppercase;
+
-
    color: rgba(0,0,0,0.8);
+
position: relative;
position: relative;
-
text-shadow: 1px 1px 2px rgba(0,0,0,0.2);
+
-moz-box-sizing: border-box;
 +
box-sizing: border-box;
 +
margin: 0;
 +
padding: 0;
 +
list-style: none;
}
}
-
h2.ss-subtitle:before{
+
 
-
width: 4px;
+
html,
-
height: 40px;
+
body {
-
background: rgba(17,17,22,0.8);
+
height: 100%;
-
content: '';
+
-
position: absolute;
+
-
right: 75%;
+
-
margin-right: -4px;
+
-
bottom: -4px;
+
-
-moz-border-radius: 2px 2px 0px 0px;
+
-
-webkit-border-radius: 2px 2px 0px 0px;
+
-
border-radius: 2px 2px 0px 0px;
+
}
}
-
h2.ss-subtitle:after{
+
 
-
width: 25%;
+
body {
-
height: 0px;
+
background: #52be7f;
-
border-bottom: 4px dotted rgba(17,17,22,0.8);
+
color: #fff;
-
content: '';
+
-
position: absolute;
+
-
right: 50%;
+
-
margin-right: -1px;
+
-
bottom: -4px;
+
}
}
-
.ss-links{
+
 
-
position: fixed;
+
.component {
-
left: 10px;
+
position: relative;
-
top: 166px;
+
margin-bottom: 3em;
-
width: 40%;
+
height: 15em;
-
z-index: 100;
+
background: rgba(0,0,0,0.05);
 +
font-family: 'Lato', Arial, sans-serif;
}
}
-
.ss-links a{
+
 
-
background: rgba(0,0,0,0.2);
+
@media only screen and (max-width: 620px) {
-
font-size: 16px;
+
.no-csstransforms li {
-
width: 40px;
+
width: 4em;
-
height: 40px;
+
height: 4em;
-
line-height: 40px;
+
line-height: 4em;
-
margin: 5px;
+
}
-
float: left;
+
-
border-radius: 50%;
+
-
display: block;
+
-
text-align: center;
+
-
-webkit-transition: background 0.2s linear;
+
-
-moz-transition: background 0.2s linear;
+
-
-o-transition: background 0.2s linear;
+
-
-ms-transition: background 0.2s linear;
+
-
transition: background 0.2s linear;
+
}
}
-
.ss-links a:hover{
+
 
-
background: rgba(0,0,0,0.4);
+
@media only screen and (max-width: 500px) {
 +
.no-ccstransforms .cn-wrapper {
 +
padding: .5em;
 +
}
 +
 
 +
.no-csstransforms .cn-wrapper li {
 +
width: 4em;
 +
height: 4em;
 +
font-size: .9em;
 +
line-height: 4em;
 +
}
}
}
-
.ss-container{
+
 
-
    width: 100%;
+
@media only screen and (max-width: 480px) {
-
    position: relative;
+
.csstransforms .cn-wrapper {
-
    text-align: left;
+
font-size: .68em;
-
    float: left;
+
}
-
overflow: hidden;
+
 
-
padding-bottom: 500px;
+
.cn-button {
 +
font-size: 1em;
 +
}
}
}
-
.ss-container h2{
+
 
-
    font-size: 40px;
+
@media only screen and (max-width:420px) {
-
    text-transform: uppercase;
+
.no-csstransforms .cn-wrapper li {
-
    color: rgba(78,84,123,0.2);
+
width: 100%;
-
text-shadow: 0px 1px 1px #fff;
+
height: 3em;
-
padding: 20px 0px;
+
line-height: 3em;
-
}
+
}
-
.ss-container:before{
+
-
    position: absolute;
+
-
    width: 4px;
+
-
    background: rgba(17,17,22,0.8);
+
-
    top: 0px;
+
-
    left: 50%;
+
-
margin-left: -2px;
+
-
    content: '';
+
-
    height: 100%;
+
-
}
+
-
.ss-row{
+
-
    width: 100%;
+
-
    clear: both;
+
-
    float: left;
+
-
    position: relative;
+
-
    padding: 30px 0;
+
-
}
+
-
.ss-left, .ss-right{
+
-
    float: left;
+
-
    width: 48%;
+
-
    position: relative;
+
-
}
+
-
.ss-right{
+
-
    padding-left: 2%;
+
-
}
+
-
.ss-left{
+
-
    text-align: right;
+
-
    float: left;
+
-
    padding-right: 2%;
+
-
}
+
-
.ss-circle{
+
-
    border-radius: 50%;
+
-
    overflow: hidden;
+
-
    display: block;
+
-
    text-indent: -9000px;
+
-
    text-align: left;
+
-
    -webkit-box-shadow:
+
-
0px 2px 5px rgba(0,0,0,0.7) inset,
+
-
0px 0px 0px 12px rgba(61,64,85,0.3);
+
-
    -moz-box-shadow:  
+
-
0px 2px 5px rgba(0,0,0,0.7) inset,
+
-
0px 0px 0px 12px rgba(61,64,85,0.3);
+
-
    box-shadow:
+
-
0px 2px 5px rgba(0,0,0,0.7) inset,
+
-
0px 0px 0px 12px rgba(61,64,85,0.3);
+
-
background-size: cover;
+
-
background-color: #f0f0f0;
+
-
background-repeat: no-repeat;
+
-
background-position: center center;
+
-
position: static;
+
-
}
+
-
.ss-small .ss-circle{
+
-
width: 100px;
+
-
height: 100px;
+
-
}
+
-
.ss-medium .ss-circle{
+
-
width: 200px;
+
-
height: 200px;
+
-
}
+
-
.ss-large .ss-circle{
+
-
width: 300px;
+
-
height: 300px;
+
-
}
+
-
.ss-circle-deco:before{
+
-
width: 29%;
+
-
height: 0px;
+
-
border-bottom: 5px dotted #ddd;
+
-
border-bottom: 5px dotted rgba(17, 17, 22, 0.3);
+
-
-webkit-box-shadow: 0px 1px 1px #fff;
+
-
-moz-box-shadow: 0px 1px 1px #fff;
+
-
box-shadow: 0px 1px 1px #fff;
+
-
position: absolute;
+
-
top: 50%;
+
-
content: '';
+
-
margin-top: -3px;
+
-
}
+
-
.ss-circle-deco:after{
+
-
width: 0px;
+
-
height: 0px;
+
-
border-top: 10px solid transparent;
+
-
    border-bottom: 10px solid transparent;
+
-
content: '';
+
-
position: absolute;
+
-
top: 50%;
+
-
margin-top: -10px;
+
-
}
+
-
.ss-left .ss-circle-deco:before{
+
-
    right: 2%; 
+
-
}
+
-
.ss-right .ss-circle-deco:before{
+
-
    left: 2%; 
+
-
}
+
-
.ss-left .ss-circle-deco:after{
+
-
right: 0;
+
-
border-right: 10px solid rgba(17,17,22,0.8);
+
-
}
+
-
.ss-right .ss-circle-deco:after{
+
-
left: 0;
+
-
border-left: 10px solid rgba(17,17,22,0.8);
+
-
}
+
-
.ss-left .ss-circle{
+
-
    float: right;
+
-
    margin-right: 30%;
+
-
}
+
-
.ss-right .ss-circle{
+
-
    float: left;
+
-
    margin-left: 30%;
+
-
}
+
-
.ss-container h3{
+
-
    margin-top: 34px;
+
-
padding: 10px 15px;
+
-
background: rgba(26, 27, 33, 0.6);
+
-
text-shadow: 1px 1px 1px rgba(26, 27, 33, 0.8)
+
-
}
+
-
.ss-container .ss-medium h3{
+
-
margin-top: 82px;
+
-
}
+
-
.ss-container .ss-large h3{
+
-
margin-top: 133px;
+
-
}
+
-
.ss-container .ss-left h3{
+
-
border-right: 5px solid rgba(164,166,181,0.8);
+
-
}
+
-
.ss-container .ss-right h3{
+
-
border-left: 5px solid rgba(164,166,181,0.8);
+
-
}
+
-
.ss-container h3 span{
+
-
    color: rgba(255,255,255,0.8);
+
-
    font-size: 13px;
+
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-
    </head>
 
-
    <body>
 
-
        <p>&nbsp;</p>
 
-
        <div class="container">
 
-
            <h2 class="ss-subtitle">RESULTS</h2>
+
</head>
-
<div id="ss-links" class="ss-links">
+
<body>
-
<a href="#Brain">Brain</a>
+
<div class="container">
-
<a href="#Liver">Liver</a>
+
<header>
-
<a href="#T cell">T cell</a>
+
<h1>Liver: <span>For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b.
 +
Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.</span></h1>
 +
</header>
 +
<div>
 +
<a><img id="Logo";width="750px"; height="500px" src="https://static.igem.org/mediawiki/2013/5/54/NJU_China_Team.JPG" style="position:absolute; left:220px; top:900px; "/></a>
 +
</div>
-
</div>
+
             <!--Start NavBar-->
-
             <div id="ss-container" class="ss-container">
+
<ul id="nav">  
-
                <div class="ss-row">
+
    <li><a href="https://2013.igem.org/Team:NJU_China">Home</a></li>
-
                    <div class="ss-left">
+
-
                        <h2 id="Brain">Brain</h2>
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Team">Team</a></li>  
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
                <div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
<a>Liver:</a>
+
-
<span>For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b.</BR>
+
-
Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.</span>
+
-
                         
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
<a>Results for liver targeting:</a>
+
-
<span>To produce the exosomes that have pre-S1 on their surface for liver targeting, we first transfected the exosome-producing cells, HEK 293T cells, with the plasmid encoding the fusion protein of lamp 2b and pre-S1 peptide.</span>
+
-
                         
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row">
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a></li>  
-
                    <div class="ss-left">
+
-
                        <h2 id="Liver">Liver</h2>
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Parts">Parts</a></li>
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
                <div class="ss-row ss-medium">
+
-
                    <div class="ss-rihgt">
+
-
                        <a class="ss-circle ss-circle-2">Week2 (14th-18th, April)</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
                        <h3>
+
-
<a>siRNA screening (Failed)</a>
+
-
<span>14th: We extracted plasmids and cultured the 293t cells.</span>
+
-
<span>15th: We transfected plasmids into 293t cells.</span>
+
-
<span>16th: We collected cells and preserved it in Trizol.</span>
+
-
<span>17th: We extracted RNA, then did RT-PCR with the RNA.</span>
+
-
<span>18th: We did qPCR with the cDNA we got on 17th April.</span>
+
-
<span>21th: We reexamined the concentration of RNA, and redid qPCR.</span>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a class="ss-circle ss-circle-3">Week3 (27th, April – 1st, May)</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
<a> siRNA screening (Failed)</a>
+
-
<span>27th: We cultured the 293t cells in a 12-well plate.</span>
+
-
<span>28th: We transfected 293t cells.</span>
+
-
<span>29th: We collected cells and extracted RNA from it.</span>
+
-
<span>30th: We did RT-qPCR with the RNA we extracted on 29th April.</span>
+
-
<span>1st: We did qPCR with the cDNA we got on 30th April.</span>
+
-
                        </h3>
+
-
                    </div>
+
-
                   
+
-
<div class="ss-row">
+
-
                    <div class="ss-left">
+
-
                        <h2 id="T cell">T cell</h2>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
                <div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a class="ss-circle ss-circle-4">Week4 (8th-13th, May)</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
                        <h3>
+
-
<a> siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)</a>
+
-
<span>8th: We extracted plasmids of 3 kinds of siRNA.</span>
+
-
<span>9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.</span>
+
-
<span>10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.</span>
+
-
<span>11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.</span>
+
-
<span>12th: We extracted RNA from cells and exosomes.</span>
+
-
<span>13th: We did RT-PCR and qPCR.</span>
+
-
                        </h3>
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Modeling">Modeling</a></li>  
-
                    </div>
+
   
-
                    <div class="ss-right">
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Notebook</a></li>  
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-15">Scrollbar Visibility with jScrollPane</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-16">Multi-level Photo Map</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>September 27, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Multi-level Photo Map</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-large">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-17">Responsive Image Gallery with Thumbnail Carousel</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>September 20, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Responsive Image Gallery with Thumbnail Carousel</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>September 12, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Elastislide - A Responsive jQuery Carousel Plugin</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-18">Elastislide - A Responsive jQuery Carousel Plugin</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-19">Slicebox - A fresh 3D image slider with graceful fallback </a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>September 5, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Slicebox - A fresh 3D image slider with graceful fallback </a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row">
+
-
                    <div class="ss-left">
+
-
                        <h2 id="august">August</h2>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-large">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>August 30, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Automatic Image Montage with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-20">Automatic Image Montage with jQuery</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-21">Image Zoom Tour with jQuery</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>August 23, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Image Zoom Tour with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>August 16, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Circular Content Carousel with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-22">Circular Content Carousel with jQuery</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-large">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-23">Portfolio Image Navigation with jQuery</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>August 9, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Portfolio Image Navigation with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>August 4, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Expanding Fullscreen Grid Portfolio</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-24">Expanding Fullscreen Grid Portfolio</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row">
+
-
                    <div class="ss-left">
+
-
                        <h2 id="july">July</h2>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-25">Content Rotator with jQuery</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>July 29, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Content Rotator with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-large">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>July 22, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Vertical Sliding Accordion with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com" class="ss-circle ss-circle-26">Vertical Sliding Accordion with jQuery</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-medium">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com" class="ss-circle ss-circle-27">Animated Text and Icon Menu with jQuery</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>July 12, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Animated Text and Icon Menu with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
<h3>
+
-
                            <span>July 5, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Fullscreen Slideshow with HTML5 Audio and jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-30">Fullscreen Slideshow with HTML5 Audio and jQuery</a>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-large">
+
-
                    <div class="ss-left">
+
-
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-28">Sliding Background Image Menu with jQuery</a>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h3>
+
-
                            <span>July 3, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Sliding Background Image Menu with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row">
+
-
                    <div class="ss-left">
+
-
                        <h2 id="june">June</h2>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
                        <h2>2013</h2>
+
-
                    </div>
+
-
                </div>
+
-
<div class="ss-row ss-small">
+
-
                    <div class="ss-left">
+
-
                      <h3>
+
-
                            <span>June 9, 2013</span>
+
-
                            <a href="http://sc.chinaz.com/">Grid Navigation Effects with jQuery</a>
+
-
                        </h3>
+
-
                    </div>
+
-
                    <div class="ss-right">
+
-
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-29">Grid Navigation Effects with jQuery</a>
+
-
                    </div>
+
-
                </div>
+
-
            </div>
+
-
        </div>
+
-
        <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
+
-
<script language="Javascript">
+
-
/*
+
-
* jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
+
-
*
+
-
* Uses the built in easing capabilities added In jQuery 1.1
+
-
* to offer multiple easing options
+
-
*
+
-
* TERMS OF USE - jQuery Easing
+
-
*
+
-
* Open source under the BSD License.
+
-
*
+
-
* Copyright © 2008 George McGinley Smith
+
-
* All rights reserved.
+
-
*
+
-
* Redistribution and use in source and binary forms, with or without modification,
+
-
* are permitted provided that the following conditions are met:
+
-
*
+
-
* Redistributions of source code must retain the above copyright notice, this list of
+
-
* conditions and the following disclaimer.
+
-
* Redistributions in binary form must reproduce the above copyright notice, this list
+
-
* of conditions and the following disclaimer in the documentation and/or other materials
+
-
* provided with the distribution.
+
-
*
+
-
* Neither the name of the author nor the names of contributors may be used to endorse
+
-
* or promote products derived from this software without specific prior written permission.
+
-
*
+
-
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
+
-
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
+
-
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
+
-
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+
-
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
+
-
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
+
-
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
+
-
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
+
-
* OF THE POSSIBILITY OF SUCH DAMAGE.
+
-
*
+
-
*/
+
-
// t: current time, b: begInnIng value, c: change In value, d: duration
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a></li>
-
jQuery.easing['jswing'] = jQuery.easing['swing'];
+
-
jQuery.extend( jQuery.easing,
+
    <li><a href="https://2013.igem.org/Team:NJU_China/Attributions">Attributions</a></li>
-
{
+
   
-
def: 'easeOutQuad',
+
</ul>
-
swing: function (x, t, b, c, d) {
+
<!--End NavBar-->
-
//alert(jQuery.easing.default);
+
<div class="component">
-
return jQuery.easing[jQuery.easing.def](x, t, b, c, d);
+
</div>
-
},
+
<section>
-
easeInQuad: function (x, t, b, c, d) {
+
<p>Site-specific drug delivery has always been a challenge in gene therapy. It is urgently required to develop a new system to overcome the off-target effect, low efficiency and high toxicity of the currently available approaches. Recently, small interfering RNA (siRNA) is emerging as a promising therapeutic drug against a wide array of diseases. Current techniques for siRNA transfer use viruses or synthetic agents as delivery vehicles. However, these approaches are toxic and low-efficient, and more importantly, can not deliver siRNA to specific tissues and organs. </p>
-
return c*(t/=d)*t + b;
+
 
-
},
+
<p>To better deliver the siRNA and other drugs, we are trying to construct a novel system which employs MVs to encapsulate and protect siRNAs for delivery to target cells. Microvesicles (MVs) are lipid bilayer vesicles which are naturally secreted by almost all cell types, playing crucial roles in intercellular transport of bioactive molecules, including siRNAs. Given the intrinsic ability to traverse biological barriers and to naturally transport functional siRNAs between cells, MVs potentially represent a novel and exciting drug carrier for therapeutic uses. Thus, MVs derived from cells engineered to express siRNAs may be capable of delivering siRNAs to local cellular environment.</p>
-
easeOutQuad: function (x, t, b, c, d) {
+
-
return -c *(t/=d)*(t-2) + b;
+
-
},
+
-
easeInOutQuad: function (x, t, b, c, d) {
+
-
if ((t/=d/2) < 1) return c/2*t*t + b;
+
-
return -c/2 * ((--t)*(t-2) - 1) + b;
+
-
},
+
-
easeInCubic: function (x, t, b, c, d) {
+
-
return c*(t/=d)*t*t + b;
+
-
},
+
-
easeOutCubic: function (x, t, b, c, d) {
+
-
return c*((t=t/d-1)*t*t + 1) + b;
+
-
},
+
-
easeInOutCubic: function (x, t, b, c, d) {
+
-
if ((t/=d/2) < 1) return c/2*t*t*t + b;
+
-
return c/2*((t-=2)*t*t + 2) + b;
+
-
},
+
-
easeInQuart: function (x, t, b, c, d) {
+
-
return c*(t/=d)*t*t*t + b;
+
-
},
+
-
easeOutQuart: function (x, t, b, c, d) {
+
-
return -c * ((t=t/d-1)*t*t*t - 1) + b;
+
-
},
+
-
easeInOutQuart: function (x, t, b, c, d) {
+
-
if ((t/=d/2) < 1) return c/2*t*t*t*t + b;
+
-
return -c/2 * ((t-=2)*t*t*t - 2) + b;
+
-
},
+
-
easeInQuint: function (x, t, b, c, d) {
+
-
return c*(t/=d)*t*t*t*t + b;
+
-
},
+
-
easeOutQuint: function (x, t, b, c, d) {
+
-
return c*((t=t/d-1)*t*t*t*t + 1) + b;
+
-
},
+
-
easeInOutQuint: function (x, t, b, c, d) {
+
-
if ((t/=d/2) < 1) return c/2*t*t*t*t*t + b;
+
-
return c/2*((t-=2)*t*t*t*t + 2) + b;
+
-
},
+
-
easeInSine: function (x, t, b, c, d) {
+
-
return -c * Math.cos(t/d * (Math.PI/2)) + c + b;
+
-
},
+
-
easeOutSine: function (x, t, b, c, d) {
+
-
return c * Math.sin(t/d * (Math.PI/2)) + b;
+
-
},
+
-
easeInOutSine: function (x, t, b, c, d) {
+
-
return -c/2 * (Math.cos(Math.PI*t/d) - 1) + b;
+
-
},
+
-
easeInExpo: function (x, t, b, c, d) {
+
-
return (t==0) ? b : c * Math.pow(2, 10 * (t/d - 1)) + b;
+
-
},
+
-
easeOutExpo: function (x, t, b, c, d) {
+
-
return (t==d) ? b+c : c * (-Math.pow(2, -10 * t/d) + 1) + b;
+
-
},
+
-
easeInOutExpo: function (x, t, b, c, d) {
+
-
if (t==0) return b;
+
-
if (t==d) return b+c;
+
-
if ((t/=d/2) < 1) return c/2 * Math.pow(2, 10 * (t - 1)) + b;
+
-
return c/2 * (-Math.pow(2, -10 * --t) + 2) + b;
+
-
},
+
-
easeInCirc: function (x, t, b, c, d) {
+
-
return -c * (Math.sqrt(1 - (t/=d)*t) - 1) + b;
+
-
},
+
-
easeOutCirc: function (x, t, b, c, d) {
+
-
return c * Math.sqrt(1 - (t=t/d-1)*t) + b;
+
-
},
+
-
easeInOutCirc: function (x, t, b, c, d) {
+
-
if ((t/=d/2) < 1) return -c/2 * (Math.sqrt(1 - t*t) - 1) + b;
+
-
return c/2 * (Math.sqrt(1 - (t-=2)*t) + 1) + b;
+
-
},
+
-
easeInElastic: function (x, t, b, c, d) {
+
-
var s=1.70158;var p=0;var a=c;
+
-
if (t==0) return b;  if ((t/=d)==1) return b+c;  if (!p) p=d*.3;
+
-
if (a < Math.abs(c)) { a=c; var s=p/4; }
+
-
else var s = p/(2*Math.PI) * Math.asin (c/a);
+
-
return -(a*Math.pow(2,10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )) + b;
+
-
},
+
-
easeOutElastic: function (x, t, b, c, d) {
+
-
var s=1.70158;var p=0;var a=c;
+
-
if (t==0) return b;  if ((t/=d)==1) return b+c;  if (!p) p=d*.3;
+
-
if (a < Math.abs(c)) { a=c; var s=p/4; }
+
-
else var s = p/(2*Math.PI) * Math.asin (c/a);
+
-
return a*Math.pow(2,-10*t) * Math.sin( (t*d-s)*(2*Math.PI)/p ) + c + b;
+
-
},
+
-
easeInOutElastic: function (x, t, b, c, d) {
+
-
var s=1.70158;var p=0;var a=c;
+
-
if (t==0) return b;  if ((t/=d/2)==2) return b+c;  if (!p) p=d*(.3*1.5);
+
-
if (a < Math.abs(c)) { a=c; var s=p/4; }
+
-
else var s = p/(2*Math.PI) * Math.asin (c/a);
+
-
if (t < 1) return -.5*(a*Math.pow(2,10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )) + b;
+
-
return a*Math.pow(2,-10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )*.5 + c + b;
+
-
},
+
-
easeInBack: function (x, t, b, c, d, s) {
+
-
if (s == undefined) s = 1.70158;
+
-
return c*(t/=d)*t*((s+1)*t - s) + b;
+
-
},
+
-
easeOutBack: function (x, t, b, c, d, s) {
+
-
if (s == undefined) s = 1.70158;
+
-
return c*((t=t/d-1)*t*((s+1)*t + s) + 1) + b;
+
-
},
+
-
easeInOutBack: function (x, t, b, c, d, s) {
+
-
if (s == undefined) s = 1.70158;
+
-
if ((t/=d/2) < 1) return c/2*(t*t*(((s*=(1.525))+1)*t - s)) + b;
+
-
return c/2*((t-=2)*t*(((s*=(1.525))+1)*t + s) + 2) + b;
+
-
},
+
-
easeInBounce: function (x, t, b, c, d) {
+
-
return c - jQuery.easing.easeOutBounce (x, d-t, 0, c, d) + b;
+
-
},
+
-
easeOutBounce: function (x, t, b, c, d) {
+
-
if ((t/=d) < (1/2.75)) {
+
-
return c*(7.5625*t*t) + b;
+
-
} else if (t < (2/2.75)) {
+
-
return c*(7.5625*(t-=(1.5/2.75))*t + .75) + b;
+
-
} else if (t < (2.5/2.75)) {
+
-
return c*(7.5625*(t-=(2.25/2.75))*t + .9375) + b;
+
-
} else {
+
-
return c*(7.5625*(t-=(2.625/2.75))*t + .984375) + b;
+
-
}
+
-
},
+
-
easeInOutBounce: function (x, t, b, c, d) {
+
-
if (t < d/2) return jQuery.easing.easeInBounce (x, t*2, 0, c, d) * .5 + b;
+
-
return jQuery.easing.easeOutBounce (x, t*2-d, 0, c, d) * .5 + c*.5 + b;
+
-
}
+
-
});
+
-
/*
+
<p>To achieve site-specific siRNA delivery, we will express targeting protein onto the surface of MVs and the modified MVs will, in theory, have the ability to target specific tissues and organs. By transfecting the producer cells with siRNA plasmids and collect MVs, we will fill the MVs with therapeutic siRNAs.</p>
-
*
+
-
* TERMS OF USE - EASING EQUATIONS
+
-
*
+
-
* Open source under the BSD License.
+
-
*
+
-
* Copyright © 2001 Robert Penner
+
-
* All rights reserved.
+
-
*
+
-
* Redistribution and use in source and binary forms, with or without modification,  
+
-
* are permitted provided that the following conditions are met:
+
-
*
+
-
* Redistributions of source code must retain the above copyright notice, this list of
+
-
* conditions and the following disclaimer.
+
-
* Redistributions in binary form must reproduce the above copyright notice, this list
+
-
* of conditions and the following disclaimer in the documentation and/or other materials
+
-
* provided with the distribution.
+
-
*
+
-
* Neither the name of the author nor the names of contributors may be used to endorse
+
-
* or promote products derived from this software without specific prior written permission.
+
-
*
+
-
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
+
-
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
+
-
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
+
-
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+
-
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
+
-
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
+
-
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
+
-
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
+
-
* OF THE POSSIBILITY OF SUCH DAMAGE.
+
-
*
+
-
*/       
+
-
</script>
+
-
<script type="text/javascript">
+
-
$(function() {
+
-
var $sidescroll = (function() {
+
<p>Our modified MVs are just like the “biomissile”, which can be delivered to specific cells and destroy target mRNAs. Our project will open up avenues for therapeutic applications of MVs as biomissile.</p>
-
+
</section>
-
// the row elements
+
</div><!-- /container -->
-
var $rows = $('#ss-container > div.ss-row'),
+
-
// we will cache the inviewport rows and the outside viewport rows
+
-
$rowsViewport, $rowsOutViewport,
+
-
// navigation menu links
+
-
$links = $('#ss-links > a'),
+
-
// the window element
+
-
$win = $(window),
+
-
// we will store the window sizes here
+
-
winSize = {},
+
-
// used in the scroll setTimeout function
+
-
anim = false,
+
-
// page scroll speed
+
-
scollPageSpeed = 2000 ,
+
-
// page scroll easing
+
-
scollPageEasing = 'easeInOutExpo',
+
-
// perspective?
+
-
hasPerspective = false,
+
-
+
-
perspective = hasPerspective && Modernizr.csstransforms3d,
+
-
// initialize function
+
-
init = function() {
+
-
+
-
// get window sizes
+
-
getWinSize();
+
-
// initialize events
+
-
initEvents();
+
-
// define the inviewport selector
+
-
defineViewport();
+
-
// gets the elements that match the previous selector
+
-
setViewportRows();
+
-
// if perspective add css
+
-
if( perspective ) {
+
-
$rows.css({
+
-
'-webkit-perspective' : 600,
+
-
'-webkit-perspective-origin' : '50% 0%'
+
-
});
+
-
}
+
-
// show the pointers for the inviewport rows
+
-
$rowsViewport.find('a.ss-circle').addClass('ss-circle-deco');
+
-
// set positions for each row
+
-
placeRows();
+
-
+
-
},
+
-
// defines a selector that gathers the row elems that are initially visible.
+
-
// the element is visible if its top is less than the window's height.
+
-
// these elements will not be affected when scrolling the page.
+
-
defineViewport = function() {
+
-
+
-
$.extend( $.expr[':'], {
+
-
+
-
inviewport : function ( el ) {
+
-
if ( $(el).offset().top < winSize.height ) {
+
-
return true;
+
-
}
+
-
return false;
+
-
}
+
-
+
-
});
+
-
+
-
},
+
-
// checks which rows are initially visible
+
-
setViewportRows = function() {
+
-
+
-
$rowsViewport = $rows.filter(':inviewport');
+
-
$rowsOutViewport = $rows.not( $rowsViewport )
+
-
+
-
},
+
-
// get window sizes
+
-
getWinSize = function() {
+
-
+
-
winSize.width = $win.width();
+
-
winSize.height = $win.height();
+
-
+
-
},
+
-
// initialize some events
+
-
initEvents = function() {
+
-
+
-
// navigation menu links.
+
-
// scroll to the respective section.
+
-
$links.on( 'click.Scrolling', function( event ) {
+
-
+
-
// scroll to the element that has id = menu's href
+
-
$('html, body').stop().animate({
+
-
scrollTop: $( $(this).attr('href') ).offset().top
+
-
}, scollPageSpeed, scollPageEasing );
+
-
+
-
return false;
+
-
+
-
});
+
-
+
-
$(window).on({
+
-
// on window resize we need to redefine which rows are initially visible (this ones we will not animate).
+
-
'resize.Scrolling' : function( event ) {
+
-
+
-
// get the window sizes again
+
-
getWinSize();
+
-
// redefine which rows are initially visible (:inviewport)
+
-
setViewportRows();
+
-
// remove pointers for every row
+
-
$rows.find('a.ss-circle').removeClass('ss-circle-deco');
+
-
// show inviewport rows and respective pointers
+
-
$rowsViewport.each( function() {
+
-
+
-
$(this).find('div.ss-left')
+
-
  .css({ left  : '0%' })
+
-
  .end()
+
-
  .find('div.ss-right')
+
-
  .css({ right  : '0%' })
+
-
  .end()
+
-
  .find('a.ss-circle')
+
-
  .addClass('ss-circle-deco');
+
-
+
-
});
+
-
+
-
},
+
-
// when scrolling the page change the position of each row
+
-
'scroll.Scrolling' : function( event ) {
+
-
+
-
// set a timeout to avoid that the
+
-
// placeRows function gets called on every scroll trigger
+
-
if( anim ) return false;
+
-
anim = true;
+
-
setTimeout( function() {
+
-
+
-
placeRows();
+
-
anim = false;
+
-
+
-
}, 10 );
+
-
+
-
}
+
-
});
+
-
+
-
},
+
-
// sets the position of the rows (left and right row elements).
+
-
// Both of these elements will start with -50% for the left/right (not visible)
+
-
// and this value should be 0% (final position) when the element is on the
+
-
// center of the window.
+
-
placeRows = function() {
+
-
+
-
// how much we scrolled so far
+
-
var winscroll = $win.scrollTop(),
+
-
// the y value for the center of the screen
+
-
winCenter = winSize.height / 2 + winscroll;
+
-
+
-
// for every row that is not inviewport
+
-
$rowsOutViewport.each( function(i) {
+
-
+
-
var $row = $(this),
+
-
// the left side element
+
-
$rowL = $row.find('div.ss-left'),
+
-
// the right side element
+
-
$rowR = $row.find('div.ss-right'),
+
-
// top value
+
-
rowT = $row.offset().top;
+
-
+
-
// hide the row if it is under the viewport
+
-
if( rowT > winSize.height + winscroll ) {
+
-
+
-
if( perspective ) {
+
-
+
-
$rowL.css({
+
-
'-webkit-transform' : 'translate3d(-75%, 0, 0) rotateY(-90deg) translate3d(-75%, 0, 0)',
+
-
'opacity' : 0
+
-
});
+
-
$rowR.css({
+
-
'-webkit-transform' : 'translate3d(75%, 0, 0) rotateY(90deg) translate3d(75%, 0, 0)',
+
-
'opacity' : 0
+
-
});
+
-
+
-
}
+
-
else {
+
-
+
-
$rowL.css({ left : '-50%' });
+
-
$rowR.css({ right : '-50%' });
+
-
+
-
}
+
-
+
-
}
+
-
// if not, the row should become visible (0% of left/right) as it gets closer to the center of the screen.
+
-
else {
+
-
+
-
// row's height
+
-
var rowH = $row.height(),
+
-
// the value on each scrolling step will be proporcional to the distance from the center of the screen to its height
+
-
factor = ( ( ( rowT + rowH / 2 ) - winCenter ) / ( winSize.height / 2 + rowH / 2 ) ),
+
-
// value for the left / right of each side of the row.
+
-
// 0% is the limit
+
-
val = Math.max( factor * 50, 0 );
+
-
+
-
if( val <= 0 ) {
+
-
+
-
// when 0% is reached show the pointer for that row
+
-
if( !$row.data('pointer') ) {
+
-
+
-
$row.data( 'pointer', true );
+
-
$row.find('.ss-circle').addClass('ss-circle-deco');
+
-
+
-
}
+
-
+
-
}
+
-
else {
+
-
+
-
// the pointer should not be shown
+
-
if( $row.data('pointer') ) {
+
-
+
-
$row.data( 'pointer', false );
+
-
$row.find('.ss-circle').removeClass('ss-circle-deco');
+
-
+
-
}
+
-
+
-
}
+
-
+
-
// set calculated values
+
-
if( perspective ) {
+
-
+
-
var t = Math.max( factor * 75, 0 ),
+
-
r = Math.max( factor * 90, 0 ),
+
-
o = Math.min( Math.abs( factor - 1 ), 1 );
+
-
+
-
$rowL.css({
+
-
'-webkit-transform' : 'translate3d(-' + t + '%, 0, 0) rotateY(-' + r + 'deg) translate3d(-' + t + '%, 0, 0)',
+
-
'opacity' : o
+
-
});
+
-
$rowR.css({
+
-
'-webkit-transform' : 'translate3d(' + t + '%, 0, 0) rotateY(' + r + 'deg) translate3d(' + t + '%, 0, 0)',
+
-
'opacity' : o
+
-
});
+
-
+
-
}
+
-
else {
+
-
+
-
$rowL.css({ left : - val + '%' });
+
-
$rowR.css({ right : - val + '%' });
+
-
+
-
}
+
-
+
-
}
+
-
+
-
});
+
-
+
-
};
+
-
+
-
return { init : init };
+
-
+
-
})();
+
-
+
-
$sidescroll.init();
+
-
+
-
});
+
-
</script>
+
-
    </body>
+
</body>
</html>
</html>

Revision as of 12:43, 26 September 2013

<!DOCTYPE html> NJU_China

Liver: For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b. Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.

Site-specific drug delivery has always been a challenge in gene therapy. It is urgently required to develop a new system to overcome the off-target effect, low efficiency and high toxicity of the currently available approaches. Recently, small interfering RNA (siRNA) is emerging as a promising therapeutic drug against a wide array of diseases. Current techniques for siRNA transfer use viruses or synthetic agents as delivery vehicles. However, these approaches are toxic and low-efficient, and more importantly, can not deliver siRNA to specific tissues and organs.

To better deliver the siRNA and other drugs, we are trying to construct a novel system which employs MVs to encapsulate and protect siRNAs for delivery to target cells. Microvesicles (MVs) are lipid bilayer vesicles which are naturally secreted by almost all cell types, playing crucial roles in intercellular transport of bioactive molecules, including siRNAs. Given the intrinsic ability to traverse biological barriers and to naturally transport functional siRNAs between cells, MVs potentially represent a novel and exciting drug carrier for therapeutic uses. Thus, MVs derived from cells engineered to express siRNAs may be capable of delivering siRNAs to local cellular environment.

To achieve site-specific siRNA delivery, we will express targeting protein onto the surface of MVs and the modified MVs will, in theory, have the ability to target specific tissues and organs. By transfecting the producer cells with siRNA plasmids and collect MVs, we will fill the MVs with therapeutic siRNAs.

Our modified MVs are just like the “biomissile”, which can be delivered to specific cells and destroy target mRNAs. Our project will open up avenues for therapeutic applications of MVs as biomissile.

Retrieved from "http://2013.igem.org/Team:NJU_China/Project/Brain"