Team:SUSTC-Shenzhen-A/Project
From 2013.igem.org
(Difference between revisions)
(→our design) |
(→our design) |
||
Line 40: | Line 40: | ||
'''Cell A:''' | '''Cell A:''' | ||
Constant expressions: | Constant expressions: | ||
- | '''LuxR'''(with C6HSL, activate pLux, express Zeo-r): promoter J23100 and rbs B0034 | + | '''LuxR'''(with C6HSL, activate pLux, express Zeo-r): promoter J23100 and rbs B0034</p> |
- | '''araC'''(with arabinose, activate LasI, tetA): promoter J23100 and rbs B0034 | + | <p>'''araC'''(with arabinose, activate LasI, tetA): promoter J23100 and rbs B0034</p> |
- | '''mCherry '''(red fluorescent protein labeled cell A): promoter pCon, rbs | + | <p>'''mCherry '''(red fluorescent protein labeled cell A): promoter pCon, rbs </p> |
- | Adjustable expressions: | + | <p>Adjustable expressions:</p> |
- | '''Zeo-r,''' adjusted by C6HSL, produced by LuxI from '''cell B''', depends on cell B density and IPTG concentration | + | <p>'''Zeo-r,''' adjusted by C6HSL, produced by LuxI from '''cell B''', depends on cell B density and IPTG concentration</p> |
- | '''LasI:''' adjusted by arabinose, LasI produces C12HSL, control cell B | + | <p>'''LasI:''' adjusted by arabinose, LasI produces C12HSL, control cell B</p> |
- | '''tetA:''' adjusted by arabinose | + | <p>'''tetA:''' adjusted by arabinose</p> |
- | '''Arabinose, Zeocin, Nickel''' concentration are controlled by you | + | <p>'''Arabinose, Zeocin, Nickel''' concentration are controlled by you</p> |
- | '''C12SHL '''concentration: controlled by cell A concentration and arabinose | + | <p>'''C12SHL '''concentration: controlled by cell A concentration and arabinose</p> |
- | '''Nickel '''either kill cell or slow cell growth: '''tetA''' increase sensitivity of '''cell B '''to''' Nickel''': | + | <p>'''Nickel '''either kill cell or slow cell growth: '''tetA''' increase sensitivity of '''cell B '''to''' Nickel''':</p> |
- | zeocin either kill cell or slow cell growth:Zeo-r decreases sensitivity of''' cell B''' to''' zeocin''' | + | <p>zeocin either kill cell or slow cell growth:Zeo-r decreases sensitivity of''' cell B''' to''' zeocin'''</p> |
- | '''-asv''': amino acid sequence that increase response time of LuxI, tetA and Zeo-r to regulation | + | <p>'''-asv''': amino acid sequence that increase response time of LuxI, tetA and Zeo-r to regulation</p> |
- | pMB1 and p15A: control plasmid replication in E coli | + | pMB1 and p15A: control plasmid replication in E coli</p> |
- | Kan and Cam(r): with antibiotics Kanamycin and chloramphenicol, prevent lost of plasmids | + | <p>Kan and Cam(r): with antibiotics Kanamycin and chloramphenicol, prevent lost of plasmids |
+ | </p> | ||
=== Experiment process === | === Experiment process === |
Revision as of 13:47, 26 September 2013
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions | Human Practice |
---|
Contents |