Team:KIT-Kyoto/Notebook/ATF1/september

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Latest revision as of 09:19, 27 September 2013

ATF1

September 1^st

Picked 32 colonies of ATF1 transformants.

September 3^rd

Checked the colonies by colony cracking.

No appropriate plasmid DNA was detected.

Digested ATF1 and pET-15b with XhoI and Bpu1102I at 37˚C overnight.

ATF1	24µL
XhoI	1µL
Bpu1102I	1µL
BSA	1µL
NEB Buffer 2	3µL

pET-15b	33µL
XhoI	1µL
Bpu1102I	1µL
BSA	1µL
NEB Buffer 2	4µL

September 4^th

Applied pET-15b and ATF1 to the blue gel electrophoresis.

No pET-15b was detected.

ATF1 was extracted from the blue gel.

September 11^th

PCR reaction was carried out using the primer.

(Forward : CGATCCTCGAGTGGCCTGGTATTGTCATCGCGTTG

Reverse : CGATCGCTCAGCAACCAACCAAAGCCGAGGGAGTG)

Buffer	50µL
dNTP	20µL
Primer mix	1µL
Genome DNA	0.5µL
KOD-FX	2µL
H₂O	26.5µL

Electrophoresed in 1% agarose gel. Verified as ATF1.

Purified the PCR products of ATF1.

Digested ATF1 and pET-15b with XhoI and Bpu1102I at 37˚C for 2 hours.

ATF1	15.5µL
XhoI	1µL
Bpu1102I	1µL
BSA	0.5µL
NEB Buffer 2	2µL

Added 1µL of BAP to the solution of pET-15b and incubated it at 37˚C for 30 minutes.

Applied pET-15b and ATF1 to the blue gel electrophoresis.

Isolated and purified them.

Ligated pET-15b and ATF1 and transformed.

September 12th

Picked 32 colonies of ATF1 transformants.

September 13th

Checked the colonies by colony cracking.

Picked up the appropriate colonies and cultured in 3mL LB medium with ampicillin at 37˚C for 5 hours.

Miniprepped plasmid DNA.

Digested plasmid DNA with XhoI and Bpu1102I at 37˚C for 2 hours.

ATF1 into pET-15b	15.5µL
XhoI	1µL
Bpu1102I	1µL
BSA	0.5µL
NEB Buffer 2	2µL

Applied pET-15b to the agarose gel electrophoresis.

No band was detected.

Plasmid DNA was digested with XhoI and Bpu1102I at 37˚C (overnight).

ATF1 into pET-15b	15.5µL
XhoI	1µL
Bpu1102I	1µL
BSA	0.5µL
NEB Buffer 2	2µL

September 14^th

ATF1 and pET-15b were applied to electrophoresis to the agarose gel.

No band was detected.

Digested ATF1 and pET-15b with Bpu1102I at 37˚C overnight.

ATF1	26µL
Bpu1102I	1µL
Bpu1102I Buffer	3µL

pET-15b	26µL
Bpu1102I	1µL
Bpu1102I Buffer	3µL

September 15^th

Purified ATF1 and pET-15b.

Digested ATF1 and pET-15b with XhoI.

ATF1	26µL
XhoI	1µL
Bpu1102I Buffer	3µL

pET-15b	26µL
XhoI	1µL
Bpu1102I Buffer	3µL

pET-15b and ATF1 were applied to electrophoresis in the Blue gel and purified.

Ligated ATF1 and pET-15b and transformed them into E. coli cells.

September 16th

Picked 32 colonies of ATF1 transformants.

September 18th

Checked the colonies by colony cracking.

Picked up the colonies and cultured in 3mL LB medium with ampicillin at 37˚C (overnight).

September 19^th

plasmid DNA was purified and digested with XhoI and Bpu1102I at 37˚C for 2 hours.

ATF1 into pET-15b	15.5µL
XhoI	1µL
Bpu1102I	1µL
BSA	0.5µL
NEB Buffer 2	2µL

ATF1 and pET-15b were applied to electrophoresis to the agarose gel.

No band was detected.

Digested ATF1 and pET-15b with XhoI and BlpI.

ATF1	15.5µL
XhoI	1µL
BlpI	1µL
BSA	0.5µL
Buffer	2µL

pET-15b	15.5µL
XhoI	1µL
BlpI	1µL
BSA	0.5µL
Buffer	2µL

Added 1µL of BAP to the solution of pET-15b and incubated it at 37˚C for 30 minutes.

Applied pET-15b and ATF1 to the blue gel electrophoresis.

Isolated and purified them.

The ATF1 was ligated with pET-15b, and transformed into E. coli cells.

September 20^th

Picked 64 colonies of ATF1 transformants.

September 21st

Checked the colonies by colony cracking.

Picked up colonies and cultured in the LB in 3mL LB medium with ampicillin at 37˚C (overnight).

September 22nd

Miniprepped plasmid DNA and digested it with XhoI and BlpI at 37˚C for 2 hours.

Applied ATF1 into pET-15b to the agarose gel electrophoresis.

No band was detected.

@@ Line 5: / Line 5: @@
 <head>
 </head>
+</html>
 <div class="document">
    <div id="doc-left">
-<ul class="nav metro-nav-list">
+{{Template:KIT-Kyoto/menu_note}}
-   <li class="nav-header">LabNote</li>
-   <li class="">
-      <a href="#ATF1">ATF1</a>
-      <ul class="nav">
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/may">May</a></li>
-          <li><a href="">june</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/july">july</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/august">August</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/september">September</a></li>
-      </ul>
-   </li>
-   <li class="">
-      <a href="#https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF2">ATF2</a>
-      <ul class="nav">
-      </ul>
-   </li>
-   <li class="">
-      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/YJL">YJL068C</a>
-      <ul class="nav">
-      </ul>
-   </li>
-   <li class="">
-      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/growcurves">Growth Curves</a>
-      <ul class="nav">
-      </ul>
-   </li>
-  </ul>
    </div>
@@ Line 48: / Line 15: @@
 <h2>ATF1</h2>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 1<sup>st</sup></span></b></p>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked 32 colonies
-</B></FONT><SUP><FONT SIZE=4><B>st</B></FONT></SUP></SPAN></FONT></P>
+of <i>ATF1 </i>transformants.</span></p>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-colonies of </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">transformants.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>rd</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Checked
-the colonies by colony cracking.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-appropriate plasmid DNA was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b with  at 37℃ overnight.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=166 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">24ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=166 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">33ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">4ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th	</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Applied
-pET-15b and </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-pET-15b was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Extracted
-from Blue gel and purified</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>
-ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">PCR
-reaction was carried out using the primer.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=324 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
-			mix</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Genome
-			DNA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=147>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Electrophoresed
-in 1% agarose gel. Verified as </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1.</I></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Purified
-PCR products of </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1.</I></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b with Xho1 and Bpu11021 at 37℃ for 2 hours.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=167 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=96>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Added
-ul of BAP to the solution of pET-15b and incubated it at 37℃ for 30
-minutes.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Ligated
-of pET-15b with </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">and transformed.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Applied
-pET-15b and </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Isolated
-and purified them.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-th</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-colonies of </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">transformants.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-th</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Checked
-the colonies by colony cracking.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-up the appropriate colonies and cultured in the LB in 3ml
-ampicillin(+) medium at 37℃ for 5 hours.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Miniprepped
-plasmid DNA.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-plasmid DNA with Xho1 and Bpu11021 at 37℃ for 2 hours.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=206 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-			</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">into pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Applied
-pET-15b to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-band was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Plasmid
-DNA was digested with Xho1 and Bpu11021 at 37℃ (overnight).</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=206 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-			</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">into pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b were Applied</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">to electrophoresis to
-the agarose gel.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-band was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b with Bpu11021 at 37℃ overnight.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=153 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=153 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Purified
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b with Xho1.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=153 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=153 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=83>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=40>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b
-and </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-were applied to electrophoresis to the Blue gel and purified.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Ligated
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-with pET-15b and transformed them into </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>E.
-coli </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">cells.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-th</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-colonies of </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">transformants.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-th</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Checked
-the colonies by colony cracking.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-up the colonies and cultured in 3ml LB medium with ampicillin at 37℃
-(overnight).</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">plasmid
-DNA was purified and digested with Xho1 and Bpu11021 at 37℃ for 2
-hours.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=206 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-			</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">into pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu11021</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2NEB
-			Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b were applied</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">to electrophoresis to
-the agarose gel.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-band was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Digested
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-and pET-15b with Xho1 and Blp1.</FONT></SPAN></FONT></P>
-<TABLE WIDTH=206 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Blp1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<TABLE WIDTH=206 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">15.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Blp1</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">BSA</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-	<TR VALIGN=TOP>
-		<TD WIDTH=135>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
-		</TD>
-		<TD WIDTH=42>
-			<P><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2ul</FONT></SPAN></FONT></P>
-		</TD>
-	</TR>
-</TABLE>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Added
-ul of BAP to the solution of pET-15b and incubated it at 37℃ for 30
-minutes.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Applied
-pET-15b and </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Isolated
-and purified them.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1 </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">was
-ligated with pET-15b, and transformed </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>into</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>E. coli</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
-cells.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-colonies of </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1
-</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">transformants.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-st</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Checked
-the colonies by colony cracking.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Picked
-up colonies and cultured in the LB in 3ml LB medium with ampicillin
-at 37℃ (overnight).</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=4><B>September
-nd</B></FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Miniprepped
-plasmid DNA and digested it with Xho1 and Blp1 at 37℃ for 2 hours.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Applied</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>
-ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt"> into pET-15b to
-the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><FONT FACE=""><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">No
-band was detected.</FONT></SPAN></FONT></P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
-<P STYLE="margin-bottom: 0cm"><BR>
-</P>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 3<sup>rd</sup></span></b></p>
-</div>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Checked the
+colonies by colony cracking.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No appropriate
+plasmid DNA was detected.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>ATF1</i>
+and pET-15b with <i>Xho</i>I and <i>Bpu</i>1102I at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C overnight.</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>ATF1</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>33&micro;L</span></p>
+  </td>
+ </tr>
+ <tr style='height:20.15pt'>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:20.15pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt;height:20.15pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 4<sup>th&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </sup></span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied pET-15b
+and <i>ATF1</i> to the blue gel electrophoresis.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No pET-15b was
+detected.</span></p>
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i><span
+lang=EN-US style='font-size:11.0pt'> was extracted from the blue gel.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 11<sup>th</sup></span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
+carried out using the primer. </span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(Forward : CGATCCTCGAGTGGCCTGGTATTGTCATCGCGTTG</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Reverse : CGATCGCTCAGCAACCAACCAAAGCCGAGGGAGTG)</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>50&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>dNTP</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>20&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Primer mix</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Genome DNA</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>KOD-FX</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=162 valign=top style='width:121.45pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
+  </td>
+  <td width=162 valign=top style='width:121.45pt;border-top:none;border-left:
+  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26.5&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Electrophoresed in
+% agarose gel. Verified as <i>ATF1.</i></span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Purified the PCR
+products of <i>ATF1.</i></span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>ATF1</i>
+and pET-15b with <i>Xho</i>I and <i>Bpu</i>1102I at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C for 2 hours.</span></p>
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></i></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=111 valign=top style='width:83.2pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Added 1&micro;L of BAP
+to the solution of pET-15b and incubated it at 37</span><span style='font-size:
+.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:
+.0pt'>C for 30 minutes.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied pET-15b
+and <i>ATF1</i> to the blue gel electrophoresis.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Isolated and
+purified them.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligated pET-15b and
+<i>ATF1 </i>and transformed.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 12th</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked 32 colonies
+of <i>ATF1 </i>transformants.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 13th</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Checked the
+colonies by colony cracking.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up the
+appropriate colonies and cultured in 3mL LB medium with ampicillin at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C for 5 hours.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Miniprepped plasmid
+DNA.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested plasmid
+DNA with <i>Xho</i>I and <i>Bpu</i>1102I at 37</span><span style='font-size:
+.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:
+.0pt'>C for 2 hours.</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1 </span></i><span
+  lang=EN-US style='font-size:11.0pt'>into pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied pET-15b to
+the agarose gel electrophoresis.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No band was
+detected.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Plasmid DNA was
+digested with <i>Xho</i>I and <i>Bpu</i>1102I at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C (overnight).</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1 </span></i><span
+  lang=EN-US style='font-size:11.0pt'>into pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 14<sup>th</sup></span></b></p>
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i><span
+lang=EN-US style='font-size:11.0pt'> and pET-15b were applied<i> </i>to
+electrophoresis to the agarose gel.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No band was
+detected.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>ATF1</i>
+and pET-15b with <i>Bpu</i>1102I at 37</span><span style='font-size:11.0pt;
+font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C
+overnight.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 15<sup>th</sup></span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Purified <i>ATF1</i>
+and pET-15b.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>ATF1</i>
+and pET-15b with <i>Xho</i>I.</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>26&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=98 valign=top style='width:73.65pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b and <i>ATF1</i>
+were applied to electrophoresis in the Blue gel and purified.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligated <i>ATF1</i>
+and pET-15b and transformed them into <i>E. coli </i>cells.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 16th</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked 32 colonies
+of <i>ATF1 </i>transformants.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 18th</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Checked the
+colonies by colony cracking.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up the
+colonies and cultured in 3mL LB medium with ampicillin at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C (overnight).</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 19<sup>th</sup></span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>plasmid DNA was
+purified and digested with <i>Xho</i>I and<i> Bpu</i>1102I at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C for 2 hours.</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1 </span></i><span
+  lang=EN-US style='font-size:11.0pt'>into pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>NEB Buffer 2</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i><span
+lang=EN-US style='font-size:11.0pt'> and pET-15b were applied<i> </i>to
+electrophoresis to the agarose gel.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No band was
+detected.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Digested <i>ATF1</i>
+and pET-15b with <i>Xho</i>I and <i>Blp</i>I.</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Blp</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
+ style='border-collapse:collapse;border:none'>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
+  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>15.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Blp</span></i><span
+  lang=EN-US style='font-size:11.0pt'>I</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BSA</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>0.5&micro;L</span></p>
+  </td>
+ </tr>
+ <tr>
+  <td width=150 valign=top style='width:112.25pt;border:solid windowtext 1.0pt;
+  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
+  </td>
+  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
+  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+  padding:0mm 5.4pt 0mm 5.4pt'>
+  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
+  </td>
+ </tr>
+</table>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Added 1&micro;L of BAP
+to the solution of pET-15b and incubated it at 37</span><span style='font-size:
+.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:
+.0pt'>C for 30 minutes.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied pET-15b
+and <i>ATF1</i> to the blue gel electrophoresis.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Isolated and
+purified them.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The <i>ATF1 </i>was
+ligated with pET-15b, and transformed <i>into</i> <i>E. coli</i> cells.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US style='color:red'>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 20<sup>th</sup></span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked 64 colonies
+of <i>ATF1 </i>transformants.</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 21st</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Checked the
+colonies by colony cracking.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Picked up colonies
+and cultured in the LB in 3mL LB medium with ampicillin at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C (overnight).</span></p>
+<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>September 22nd</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Miniprepped plasmid
+DNA and digested it with <i>Xho</i>I and<i> Blp</i>I at 37</span><span
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>&#730;</span><span lang=EN-US
+style='font-size:11.0pt'>C for 2 hours.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Applied<i> ATF1</i>
+into pET-15b to the agarose gel electrophoresis.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>No band was
+detected.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 </div>
-</html>
+</div>