Team:IIT Delhi/Modelling

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Our model involves both activation and repression systems. Activation of <b>asr</b> promoter in acidic medium results in production of <b>GFP</b> and <b>TetR</b> proteins. Increasing tetR in the system results in repression of Tet promoter and hence reduction in the production of <b>RFP</b>.
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Parameter Description Value
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RFP maximum production rate 1mMmin-1
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RFP repression coefficient 0.1mM
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RFP degradation rate 0.0041min-1
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H+-asr cooperativity coefficient 0.4
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tetR maximum production rate 1mMmin-1
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tetR repression coefficient 0.1mM
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tetR degradation rate 0.0692min-1
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H+-asr cooperativity coefficient 0.4
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GFP maximum production rate 0.05mMmin-1
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GFP repression coefficient 0.1mM
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GFP degradation rate 0.0692min-1
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tetR-ptet cooperativity coefficient 2
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Revision as of 10:34, 27 September 2013

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Modelling and Simulation

Our model involves both activation and repression systems. Activation of asr promoter in acidic medium results in production of GFP and TetR proteins. Increasing tetR in the system results in repression of Tet promoter and hence reduction in the production of RFP. Parameter Description Value RFP maximum production rate 1mMmin-1 RFP repression coefficient 0.1mM RFP degradation rate 0.0041min-1 H+-asr cooperativity coefficient 0.4 tetR maximum production rate 1mMmin-1 tetR repression coefficient 0.1mM tetR degradation rate 0.0692min-1 H+-asr cooperativity coefficient 0.4 GFP maximum production rate 0.05mMmin-1 GFP repression coefficient 0.1mM GFP degradation rate 0.0692min-1 tetR-ptet cooperativity coefficient 2

























Feel Free to contact us at igemiitdelhi2013 at gmail dot com if you have queries; requests; suggestions et cetera.

Thanks to iGEM and IIT Delhi,
we had an awesome summer!		 Our Project was supported by and done by the students

 of IIT Delhi, India.

 This project was done as a part of iGEM:
iGEM Main Website

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