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Team:HokkaidoU Japan/Shuffling Kit/How To Use
From 2013.igem.org
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| - | < | + | <h1>How to use</h1> |
| - | < | + | <h2>What users should prepare</h2> |
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| - | To use the kit, the protein sequence you chose must have a BsaI site and | + | To use the kit, the protein sequence you chose must have specific prefix/suffix which contains a BsaI site and produce overhang. Therefore, users must design a primer to add it. To reduce time and trouble, we automated the design by creating program <a href="https://2013.igem.org/Team:HokkaidoU_Japan/Optimization/Primer_Designer">"POK-ROK Primer Designer"!</a> |
</p> | </p> | ||
Revision as of 15:12, 27 September 2013
Maestro E.coli
Optimization Kit
How to use
What users should prepare
To use the kit, the protein sequence you chose must have specific prefix/suffix which contains a BsaI site and produce overhang. Therefore, users must design a primer to add it. To reduce time and trouble, we automated the design by creating program "POK-ROK Primer Designer"!
