Team:Tianjin/Human Practice/Collaboration
From 2013.igem.org
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<p>We have helped the team BIT-China to verify the function of the RNA thermometer in BBa_K1117009. RFP is linked to this part and we observed its expression by fluorescent microscope. The result shows that SD sequence region is occluded at 30 °C, partial melting occurs at 37 °C, whereas an increase to 42 °C facilitates the mRNA–ribosome interaction owing to full liberation of the SD and AUG start codon. Our result could be seen in following pictures:</p> | <p>We have helped the team BIT-China to verify the function of the RNA thermometer in BBa_K1117009. RFP is linked to this part and we observed its expression by fluorescent microscope. The result shows that SD sequence region is occluded at 30 °C, partial melting occurs at 37 °C, whereas an increase to 42 °C facilitates the mRNA–ribosome interaction owing to full liberation of the SD and AUG start codon. Our result could be seen in following pictures:</p> | ||
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<p>Here is the structure of ROSE RNA thermometer.</p> | <p>Here is the structure of ROSE RNA thermometer.</p> | ||
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Revision as of 15:42, 27 September 2013