Team:BYU Provo/Notebook/LargePhage/Summerexp

From 2013.igem.org

(Difference between revisions)

Latest revision as of 19:55, 27 September 2013

Large Phage May - June Notebook
Large Phage March-April May-June July-August September-October	July 1 - July 15 Mutagenesis, preparations for EM and Cesium Chloride gradient Daily log
	July 16 - July 31 Another round of mutagenesis, first cesium chloride gradient Daily log
	August 1- August 15 Measuring the EM pictures, plaque tests from purified phage Daily log
	August 15- August 30 Processing CsCl gradient phage, more mutagenesis Daily log

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 {{TeamBYUProvo}}
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-| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Large Phage May - June Notebook'''</font>
+| colspan="3" | <font color="#333399" size="5" font face="Calibri">
+: '''Large Phage May - June Notebook'''</font>
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-: [[Team:BYU_Provo/Small_Phage|Overview]]
+<font size = "4">
+: <u> '''Large Phage''' </u> </font>
 : [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]]
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-<font size="3" font face="Calibri"> Text </font>
+<font size="3" font face="Calibri"> Mutagenesis, preparations for EM and Cesium Chloride gradient</font>
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-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/FT|Formating test]]
+[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/PeriodJuly1/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/Dailylog|Daily log]]
+</font>
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/PR| Progress Report]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:intropage2.tif|250px]]
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-<font size="3" font face="Calibri"> Text </font>
+<font size="3" font face="Calibri"> Another round of mutagenesis, first cesium chloride gradient </font>
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-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog|Daily log]]
+[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/PeriodJuly2/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period2/Explist|Experiment Listing]]
+</font>
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period2/PR| Progress Report]] </font>
 <br>
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+<font size="3" font face="Calibri"> Measuring the EM pictures, plaque tests from purified phage </font>
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 [[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period3/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period3/Explist|Experiment Listing]]
+</font>
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period3/PR| Progress Report]] </font>
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-| <font size="5" font face="Calibri"> '''Date''' </font>
+| <font size="5" font face="Calibri"> '''August 15- August 30''' </font>
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-<font size="3" font face="Calibri"> Add description! </font>
+<font size="3" font face="Calibri">Processing CsCl gradient phage, more mutagenesis </font>
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 [[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/PR| Progress Report]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:Example100.jpg|250px]]
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-|-
-| style="width: 18%; background-color: transparent;"|
-| <font size="5" font face="Calibri"> '''Date''' </font>
-<br>
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-<br>
-<font color="#333399" size="4" font face="Calibri">
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period5/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period5/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period5/PR| Progress Report]] </font>
-<br>
-| style="width: 20%; background-color: transparent;"| [[2.JPG|250px|center]]
 |}
-{{TeamBYUProvoFooter}}
-==July==
-===7/1/13===
-===7/3/13===
-===7/5/13===
-===7/8/13===
-Today we need to address some problems we ran into and make some plans for the future.
-. Plaque sizes aren't consistent, even though we are using the same agarose each time.
-<br>2. We haven't been able to observe plaque phenotypes staying the same through multiple generations
-<br>3. We have tried multiple liquid cultures and rounds of mutagenesis but we haven't been able to observe clearage in a flask yet.
-Good things to model--
-<br>- Change in plaque size vs. agarose concentration
-<br>- Mutant vs wild type phage under UV - graph survival rates
-We looked at a new procedure from the article "Genetic Control of Capsid Length in Bacteriophage T4" and found a much better and easier purification technique. We also read about centrifuge rotors here - http://bricker.tcnj.edu/tech/BIOL311centrifugation.html
-===7/10/13===
-===7/12/13===
-===7/15/13===
-===7/17/13===
-===7/19/13===
-===7/22/13===
-===7/24/13===
-===7/26/13===
-===7/29/13===
-===7/31/13===
-==August==
-===8/2/13===
-===8/5/13===
-===8/7/13===
-===8/9/13===
-===8/12/13===
-===8/14/13===
-===8/16/13===
-===8/19/13===
-===8/21/13===
-===8/23/13===
-===8/26/13===
-===8/28/13===
-===8/30/13===
-==September==
-===9/2/13===
-===9/4/13===
-===9/6/13===
-===9/9/13===
-===9/11/13===
-===9/13/13===
-===9/16/13===
-===9/18/13===
-===9/20/13===
-===9/23/13===
-===9/25/13===
-===9/27/13===
-===9/30/13===
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