Team:BYU Provo/Notebook/LargePhage/Summerexp

From 2013.igem.org

(Difference between revisions)

Latest revision as of 19:55, 27 September 2013

Large Phage May - June Notebook
Large Phage March-April May-June July-August September-October	July 1 - July 15 Mutagenesis, preparations for EM and Cesium Chloride gradient Daily log
	July 16 - July 31 Another round of mutagenesis, first cesium chloride gradient Daily log
	August 1- August 15 Measuring the EM pictures, plaque tests from purified phage Daily log
	August 15- August 30 Processing CsCl gradient phage, more mutagenesis Daily log

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-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/FT|Formating test]]
+[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/PeriodJuly1/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/Dailylog|Daily log]]
+</font>
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period1/PR| Progress Report]] </font>
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-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog|Daily log]]
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-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period2/Explist|Experiment Listing]]
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 [[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period3/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period3/Explist|Experiment Listing]]
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-| <font size="5" font face="Calibri"> '''Date''' </font>
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 [[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/Dailylog|Daily log]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/LargePhage/Summerexp/Period4/PR| Progress Report]] </font>
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-==July==
-===7/1/13===
-===7/3/13===
-===7/5/13===
-===7/8/13===
-Today we need to address some problems we ran into and make some plans for the future.
-. Plaque sizes aren't consistent, even though we are using the same agarose each time.
-<br>2. We haven't been able to observe plaque phenotypes staying the same through multiple generations
-<br>3. We have tried multiple liquid cultures and rounds of mutagenesis but we haven't been able to observe clearage in a flask yet.
-Good things to model--
-<br>- Change in plaque size vs. agarose concentration
-<br>- Mutant vs wild type phage under UV - graph survival rates
-We looked at a new procedure from the article "Genetic Control of Capsid Length in Bacteriophage T4" and found a much better and easier purification technique. We also read about centrifuge rotors here - http://bricker.tcnj.edu/tech/BIOL311centrifugation.html
-===7/10/13===
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-==August==
-===8/2/13===
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-==September==
-===9/2/13===
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-===9/30/13===
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