"
Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog
From 2013.igem.org
| (One intermediate revision not shown) | |||
| Line 10: | Line 10: | ||
|- valign="top" | |- valign="top" | ||
| - | | style="width: | + | | style="width: 20%; background-color: transparent;"| |
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
| - | : | + | <font size = "4"> |
| + | |||
| + | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
| Line 26: | Line 28: | ||
</font> | </font> | ||
| - | | style="width: | + | |
| + | |||
| + | | style="width: 80%; background-color: transparent;"| | ||
<font face="Calibri" size="3"> | <font face="Calibri" size="3"> | ||
<br> | <br> | ||
| - | <html> | + | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a> |
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | ||
| Line 103: | Line 107: | ||
</font> | </font> | ||
| - | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/ | + | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a> |
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a> | ||
Latest revision as of 00:04, 28 September 2013
| Phage Purification May - June Notebook: June 10 - June 23 Daily Log
| ||
|
|
6/10/13 - Prepared RNase A for future use. - Prepared T4 and T7 cultures.
6/12/13 - Began first step of purification process using phage from 6.10 Phage Preparation.
6/14/13 - Worked on updating the website.
6/17/13 - Set up CsCl gradient 6.17 CsCl Gradient - Prepared T4 and T7 cultures.
6/19/13 - Began first step of purification process using phage from 6.17 Phage Preparation. - Ran spot tests on collected band from 6.17 CsCl Gradient.
6/20/13 - Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.
6/21/13 We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.
| |
