Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

From 2013.igem.org

(Difference between revisions)
(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 27 - June 9 Daily Log''...")
 
(27 intermediate revisions not shown)
Line 4: Line 4:
{| width="100%"
{| width="100%"
-
| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 27 - June 9 Daily Log'''</font>
+
| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: June 10 - June 23 Daily Log'''</font>
<br>
<br>
Line 10: Line 10:
|- valign="top"
|- valign="top"
-
| style="width: 18%; background-color: transparent;"|
+
| style="width: 20%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Phage_Purification|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
Line 26: Line 28:
</font>
</font>
-
| style="width: 82%; background-color: transparent;"|
 
-
<font face="Calibri" size="3">
 
-
<font size="4"> '''5/29/13''' </font>
+
| style="width: 80%; background-color: transparent;"|
-
- Finished the PEG purification.
+
<font face="Calibri" size="3">
-
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
+
<br>
 +
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
 +
 +
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a>
 +
</html>
<br>
<br>
Line 40: Line 44:
<font size="4"> '''6/10/13''' </font>
<font size="4"> '''6/10/13''' </font>
-
- Prepared RNase for future use.
+
- Prepared RNase A for future use.
-
- Prepared W3110 and BL21 cultures.
+
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]]
 +
 
 +
- Prepared T4 and T7 cultures.
 +
 
 +
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]]
<br>
<br>
-
<font size="4"> '''6/3/13''' </font>
+
<font size="4"> '''6/12/13''' </font>
-
- Ran the CsCl purification step.
+
- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
-
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
+
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
-
- Next class we will attempt extracting the phage from the gradient.
+
<br>
 +
 
 +
<font size="4"> '''6/14/13''' </font>
 +
 
 +
- Worked on updating the website.
<br>
<br>
-
<font size="4"> '''6/5/13''' </font>
+
<font size="4"> '''6/17/13''' </font>
-
- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle. 
+
- Set up CsCl gradient
-
: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
+
[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]]
-
: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
+
 
-
: Placed these in the refrigerator.
+
- Prepared T4 and T7 cultures.
 +
 
 +
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]]
-
- Prepared stocks of bacteria for future use.
 
-
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
 
<br>
<br>
-
<font size="4"> '''6/7/13''' </font>
+
<font size="4"> '''6/19/13''' </font>
-
: Made 1 L of 10 X phage suspension buffer.
+
- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]].
-
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
+
 
 +
: [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]]
 +
 
 +
- Ran spot tests on collected band from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]].
 +
 
 +
:[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Exp/6.19Spot Test|6.19 Spot Test]]
 +
 
 +
 
 +
<br>
 +
 
 +
<font size="4"> '''6/20/13''' </font>
 +
 
 +
- Finished [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] by centrifuging solution at 10,000 g 15 minutes at 4<sup>◦</sup> C, removing supernatant, and suspending the pellet in phage suspension buffer.
 +
 
 +
<br>
 +
 
 +
<font size="4"> '''6/21/13''' </font>
 +
 
 +
We centrifuged the pellet at 10,000 g for 15 min at 4<sup>◦</sup> C after suspending it to clean up the walls of the tube.
 +
 
 +
<br>
</font>
</font>
 +
 +
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
 +
 +
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a>
 +
</html>
 +
|}
|}
{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 00:04, 28 September 2013


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



<< Previous Next >>


6/10/13

- Prepared RNase A for future use.

6.10 RNase A Preparation

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.

6.12 PEG Purification


6/14/13

- Worked on updating the website.


6/17/13

- Set up CsCl gradient 6.17 CsCl Gradient

- Prepared T4 and T7 cultures.

6.17 Phage Preparation



6/19/13

- Began first step of purification process using phage from 6.17 Phage Preparation.

6.19 PEG Purification

- Ran spot tests on collected band from 6.17 CsCl Gradient.

6.19 Spot Test



6/20/13

- Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.


6/21/13

We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.


<< Previous Next >>


Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Dailylog"