Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
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<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog" style="display:block;float:right;">Next >></a>
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<font size="4"> '''6/10/13''' </font>
<font size="4"> '''6/10/13''' </font>
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<font size="4"> '''6/12/13''' </font>
<font size="4"> '''6/12/13''' </font>
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- Began first step of purification process.
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10PhagePreparation|6.10 Phage Preparation]].
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
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<font size="4"> '''6/3/13''' </font>
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<font size="4"> '''6/14/13''' </font>
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- Ran the CsCl purification step.
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- Worked on updating the website.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
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<font size="4"> '''6/17/13''' </font>
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- Set up CsCl gradient
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]]
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- Prepared T4 and T7 cultures.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]]
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- Next class we will attempt extracting the phage from the gradient.
 
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<font size="4"> '''6/5/13''' </font>
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<font size="4"> '''6/19/13''' </font>
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle.
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- Began first step of purification process using phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Exp/6.17PhagePreparation|6.17 Phage Preparation]].
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]]
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: Placed these in the refrigerator.
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- Ran spot tests on collected band from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.17CsClGradient|6.17 CsCl Gradient]].
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Exp/6.19Spot Test|6.19 Spot Test]]
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- Prepared stocks of bacteria for future use.
 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
 
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<font size="4"> '''6/7/13''' </font>
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<font size="4"> '''6/20/13''' </font>
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: Made 1 L of 10 X phage suspension buffer.
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- Finished [[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Exp/6.19PEGPurification|6.19 PEG Purification]] by centrifuging solution at 10,000 g 15 minutes at 4<sup>◦</sup> C, removing supernatant, and suspending the pellet in phage suspension buffer.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
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<font size="4"> '''6/21/13''' </font>
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We centrifuged the pellet at 10,000 g for 15 min at 4<sup>◦</sup> C after suspending it to clean up the walls of the tube.
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Latest revision as of 00:04, 28 September 2013


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



<< Previous Next >>


6/10/13

- Prepared RNase A for future use.

6.10 RNase A Preparation

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.

6.12 PEG Purification


6/14/13

- Worked on updating the website.


6/17/13

- Set up CsCl gradient 6.17 CsCl Gradient

- Prepared T4 and T7 cultures.

6.17 Phage Preparation



6/19/13

- Began first step of purification process using phage from 6.17 Phage Preparation.

6.19 PEG Purification

- Ran spot tests on collected band from 6.17 CsCl Gradient.

6.19 Spot Test



6/20/13

- Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.


6/21/13

We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.


<< Previous Next >>


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