Team:BYU Provo/Notebook/Phage Purification/Springexp

From 2013.igem.org

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook'''</font>
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: '''Phage Purification May - June Notebook'''</font>
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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="5" font face="Calibri"> '''March 15 - March 31''' </font>
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<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp"><< Previous</a>
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<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Summerexp" style="display:block;float:right;">Next >></a>
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<font size="5" font face="Calibri"> '''May 1 - May 12''' </font>
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<font size="3" font face="Calibri"> This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures. </font>
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This marks the start of our small phage group! Although we are still on the background-research phase and not doing any wet-lab experiments, we are sure our work will be worthwhile. As Dr. Grose, our wonderful instructor, says "a month in the lab will say you a day in the library." After two weeks of hardcore literature search, we now have a clear idea of where our project is headed. And we are super excited to get started!
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<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period1/PR| Progress Report]] </font>
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<br>
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| style="width: 20%; background-color: transparent;"| [[File:BYUPPSpringIcon1.JPG|220px|center|link=]]
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| <font size="5" font face="Calibri"> '''May 13 - May 26''' </font>
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<font size="3" font face="Calibri"> We began performing titers to test the viability of phage after purification. We also ran a CsCl gradient, and started another round of PEG purification on T7 and T4 phage. </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period5/PR|Presentation]] </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:Winter1.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring2.JPG|220px|center]]
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| <font size="5" font face="Calibri"> '''April 1 - April 14''' </font>
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| <font size="5" font face="Calibri"> '''May 27 - June 9''' </font>
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<font size="3" font face="Calibri"> Add description of the two weeks </font>
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<font size="3" font face="Calibri"> In these weeks, the CsCl gradients that we ran gave us an idea of the location of bands for both phage types, so that when mutated phage becomes available, we will be able to purify and extract it.We have also started a new round of PEG phage purification to run through the CsCl gradient again. </font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period6/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:Winter2.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Archesutah4.jpg|220px|center]]
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| <font size="5" font face="Calibri"> '''June 15 - June 30''' </font>
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| <font size="5" font face="Calibri"> '''June 10 - June 23''' </font>
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<font size="3" font face="Calibri"> Add description of the two weeks! </font>
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<font size="3" font face="Calibri"> In these two weeks, we worked on perfecting our techniques and preparing new samples to test. We purified more T7 phage using PEG purification, and it will be ready to run through CsCl gradients in the next two weeks.</font>
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period4/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period7/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:Spring4.JPG|220px|center]]
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| <font size="5" font face="Calibri"> '''June 24 - June 30''' </font>
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<font size="3" font face="Calibri"> This week we finished a CsCl gradient of T7 phage. We successfully banded and extracted the phage. We performed a titer on the extracted phage and had a concentration high enough for an Electron Microscopy sample. We also prepared new bacteria to use with the large phage group. </font>
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<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period5/Explist|Experiment Listing]]
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<br>
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<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp"><< Previous</a>
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<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Summerexp" style="display:block;float:right;">Next >></a>
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| style="width: 20%; background-color: transparent;"| [[File:Winter3.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:archesbyu.JPG|220px|center]]

Latest revision as of 01:20, 28 September 2013


Phage Purification May - June Notebook



Phage Purification
March-April
May-June
July-August
September-October

<< Previous Next >>

May 1 - May 12


This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures.


Daily log

Experiment Listing

Progress Report


BYUPPSpringIcon1.JPG


May 13 - May 26


We began performing titers to test the viability of phage after purification. We also ran a CsCl gradient, and started another round of PEG purification on T7 and T4 phage.


Daily log

Experiment Listing

Progress Report



Spring2.JPG


May 27 - June 9


In these weeks, the CsCl gradients that we ran gave us an idea of the location of bands for both phage types, so that when mutated phage becomes available, we will be able to purify and extract it.We have also started a new round of PEG phage purification to run through the CsCl gradient again.


Daily log

Experiment Listing



Archesutah4.jpg


June 10 - June 23


In these two weeks, we worked on perfecting our techniques and preparing new samples to test. We purified more T7 phage using PEG purification, and it will be ready to run through CsCl gradients in the next two weeks.


Daily log

Experiment Listing



Spring4.JPG
June 24 - June 30


This week we finished a CsCl gradient of T7 phage. We successfully banded and extracted the phage. We performed a titer on the extracted phage and had a concentration high enough for an Electron Microscopy sample. We also prepared new bacteria to use with the large phage group.


Daily log

Experiment Listing


<< Previous Next >>


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