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Team:MSOE Milwaukee/Week5
From 2013.igem.org
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<H1 align = left>Monday</H1> | <H1 align = left>Monday</H1> | ||
| - | <p> | + | <p>As a team, we discussed how we were going to design the G-Blocks. We decided that we're going to put one gene after another, with restriction sites between them. We talked about how to design the primers to add the prefix and suffix, and we also changed our DNA sequence to remove the four restriction sites that are used in the prefix and the suffix by changing certain base pairs.</p><br> |
<H1 align = left>Tuesday</H1> | <H1 align = left>Tuesday</H1> | ||
| - | <p></p><br> | + | <p>We focused on putting the sequence together and creating the primers. The sequence was finalized and submitted to our instructor for review. We hope we can order very soon, so we can get into the lab as soon as possible. Also, we designed primers to add the prefix and the suffix to our genes. These primers will be further explored using software to make sure they will work for our purposes.</p><br> |
<H1 align = left>Wednesday</H1> | <H1 align = left>Wednesday</H1> | ||
Revision as of 20:06, 2 July 2013
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Monday
As a team, we discussed how we were going to design the G-Blocks. We decided that we're going to put one gene after another, with restriction sites between them. We talked about how to design the primers to add the prefix and suffix, and we also changed our DNA sequence to remove the four restriction sites that are used in the prefix and the suffix by changing certain base pairs.
Tuesday
We focused on putting the sequence together and creating the primers. The sequence was finalized and submitted to our instructor for review. We hope we can order very soon, so we can get into the lab as soon as possible. Also, we designed primers to add the prefix and the suffix to our genes. These primers will be further explored using software to make sure they will work for our purposes.
Wednesday
Thursday
Friday
