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Team:CSU Fort Collins/Parts
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<p>Our plan was to design our bio-bricks using Gibson Assembly and gBlocks. However we were unable to get the Gibson Assemblies to work, and we were unable to produce our parts. One big hurdle in designing our parts were that some of the sequences for the proteins we wanted to use had many restriction enzyme sites that were not compatible with the bio-brick standards. This is why we chose Gibson Assembly of gBlocks over PCRing our sequences out of the yeast genome.</p> | <p>Our plan was to design our bio-bricks using Gibson Assembly and gBlocks. However we were unable to get the Gibson Assemblies to work, and we were unable to produce our parts. One big hurdle in designing our parts were that some of the sequences for the proteins we wanted to use had many restriction enzyme sites that were not compatible with the bio-brick standards. This is why we chose Gibson Assembly of gBlocks over PCRing our sequences out of the yeast genome.</p> | ||
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Revision as of 03:12, 28 September 2013
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Parts Submitted to the Registry
Our plan was to design our bio-bricks using Gibson Assembly and gBlocks. However we were unable to get the Gibson Assemblies to work, and we were unable to produce our parts. One big hurdle in designing our parts were that some of the sequences for the proteins we wanted to use had many restriction enzyme sites that were not compatible with the bio-brick standards. This is why we chose Gibson Assembly of gBlocks over PCRing our sequences out of the yeast genome.
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