Team:KAIT Japan/Results

From 2013.igem.org

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Line 23: Line 23:
<big>1 transformation</big>
<big>1 transformation</big>
-
[[File:Figure1.jpeg‎]]
+
<br>
 +
[[File:Figure1.jpeg]]
:*Figure 1transformation of Il10recepter α  
:*Figure 1transformation of Il10recepter α  
-
[[File:Figure2.jpeg‎]]
+
[[File:Figure2.jpeg]]
:*Figure2 transformation of Il10recepter β  
:*Figure2 transformation of Il10recepter β  
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
:The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
:The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
-
[[File:Fig1.png]]
+
[[File:Fig12.png]]
:*Figure3 Il10recepter α colony PCR
:*Figure3 Il10recepter α colony PCR
[[File:Fig2.png]]
[[File:Fig2.png]]
-
‎:*Figure4 Il10recepter β colony PCR
+
‎<br>
 +
:*Figure4 Il10recepter β colony PCR
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR</big>
<big>8 colony PCR</big>
:The molecular weight of the vector is a 4000 ~ 5000bp.
:The molecular weight of the vector is a 4000 ~ 5000bp.
Line 44: Line 52:
<big>'''2 STAT3'''</big>
<big>'''2 STAT3'''</big>
:We inserted stat3 gene into vector
:We inserted stat3 gene into vector
 +
<br>
<big>1 transformation</big>
<big>1 transformation</big>
-
*Figure5 transformation of STAT3  
+
<br>
 +
[[File:Figure5.jpeg]]
 +
:*Figure5 transformation of STAT3  
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
:The molecular weight of the α chain is 2307bp.
:The molecular weight of the α chain is 2307bp.
[[File:Fig6.png]]
[[File:Fig6.png]]
:*Figure6 STAT3 colony PCR  
:*Figure6 STAT3 colony PCR  
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR
<big>8 colony PCR
:The molecular weight of the vector is a 4000 ~ 5000bp.
:The molecular weight of the vector is a 4000 ~ 5000bp.
Line 61: Line 78:
<big>'''3 GFP+HlyA'''</big>
<big>'''3 GFP+HlyA'''</big>
:We inserted GFP gene and HlyA into vecter  
:We inserted GFP gene and HlyA into vecter  
 +
<br>
<big>1 transformation</big>
<big>1 transformation</big>
-
*Figure 1transformation of GFP.
+
<br>
-
*Figure2 transformation of HlyA.
+
[[File:Figure7.jpeg]]
 +
 
 +
:*Figure7 transformation of HlyA.
<big>2 .culture on the LB nutrient medium and get colonies.</big>
<big>2 .culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>3 colony PCR</big>
<big>3 colony PCR</big>
 +
<br>
:The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
:The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
-
*Figure3 Il10recepter GFP PCR
+
[[File:HlyA1.jpg]]
-
*Figure4 Il10recepter HlyA PCR
+
:*Figure8 Il-10recepter GFP PCR
 +
[[File:GFP.png]]
 +
:*Figure9 Il-10recepter HlyA PCR
 +
<br>
<big>4 restriction enzyme processing PCR production</big>
<big>4 restriction enzyme processing PCR production</big>
 +
<br>
<big>5 infusion</big>
<big>5 infusion</big>
 +
<br>
<big>6 .transformation</big>
<big>6 .transformation</big>
 +
<br>
<big>7 culture on the LB nutrient medium and get colonies.</big>
<big>7 culture on the LB nutrient medium and get colonies.</big>
 +
<br>
<big>8 colony PCR</big>
<big>8 colony PCR</big>
 +
<br>
 +
[[File:Li.png‎ ]]
:The molecular weight of the vector is a 2000~3000bp.
:The molecular weight of the vector is a 2000~3000bp.
:We were not able to confirm the vector by colony PCR.
:We were not able to confirm the vector by colony PCR.

Latest revision as of 04:14, 28 September 2013

KAIT Japan 2013RogoTop.png

KAIT Japan2013 Kanako.png KAIT Japan 2013 iGEMRogo.png

KAIT Japan home.png Home
KAIT Japan team.png Team
KAIT Japan project.png Project
KAIT Japan parts.png Parts
KAIT Japan protocol.png Protocol
KAIT Japan notebook.png Notebook

KAIT_Japan_results.png Results

KAIT Japan safety.png Safety
KAIT Japan human practice.png Human Practice


Results

1.IL10recepter

We inserted Il10receptere gene into vecter

1 transformation
Figure1.jpeg

  • Figure 1transformation of Il10recepter α

Figure2.jpeg

  • Figure2 transformation of Il10recepter β

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp

Fig12.png

  • Figure3 Il10recepter α colony PCR

Fig2.png ‎

  • Figure4 Il10recepter β colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


2 STAT3

We inserted stat3 gene into vector


1 transformation
Figure5.jpeg

  • Figure5 transformation of STAT3

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 2307bp.

Fig6.png

  • Figure6 STAT3 colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


3 GFP+HlyA

We inserted GFP gene and HlyA into vecter


1 transformation
Figure7.jpeg

  • Figure7 transformation of HlyA.

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp

HlyA1.jpg

  • Figure8 Il-10recepter GFP PCR

GFP.png

  • Figure9 Il-10recepter HlyA PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR
Li.png

The molecular weight of the vector is a 2000~3000bp.
We were not able to confirm the vector by colony PCR.






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