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Team:IIT Delhi/Project
From 2013.igem.org
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| - | valign="top"><font size="+2"> | + | valign="top"><font size="+2">Making a Comprehensive Bacterial pH sensor</font><br> |
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| + | The idea is to create a biological device that can utilize the continuous nature of transcriptional regulation (that is a more accurate representation of reality as opposed to discrete circuits) and give a gradient response. pH based response systems have not been taken up extensively in iGEM as such and offer fertile grounds for research in this field of study. A primary reason for this can be that most of the biosensors are designed to facilitate maximum growth. However, pH sensing is based on challenging this very concept.<br><br> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/2/24/Phcoli.png" align= right height= 350 width = 350> | ||
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| + | Our idea is to combine the studies exploring pH inducible promoters in molecular biology with logical systems present in the iGEM parts registry to create a Global Bacterial pH sensor akin to the Universal pH Indicator. We have made a mathematical model of our circuit and simulated it on MATLAB to observe the response characteristics. We have also indicated through a simple wet lab experiment that RFP degradation is independent of pH. | ||
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Latest revision as of 04:15, 28 September 2013
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| Making a Comprehensive Bacterial pH sensor The idea is to create a biological device that can utilize the continuous nature of transcriptional regulation (that is a more accurate representation of reality as opposed to discrete circuits) and give a gradient response. pH based response systems have not been taken up extensively in iGEM as such and offer fertile grounds for research in this field of study. A primary reason for this can be that most of the biosensors are designed to facilitate maximum growth. However, pH sensing is based on challenging this very concept. 
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Feel Free to contact us at igemiitdelhi2013 at gmail dot com if you have queries; requests; suggestions et cetera. Thanks to iGEM and IIT Delhi, we had an awesome summer! |
Our
Project was supported by and done by the students of IIT Delhi, India.  |
This
project was done as a part of iGEM: iGEM Main Website  |
