Team:UNITN-Trento/Project/Bacillus

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<i>Bacillus subtilis</i> would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a <i>B. fruity</i> home edition that exploits this principle. <br/>
<i>Bacillus subtilis</i> would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a <i>B. fruity</i> home edition that exploits this principle. <br/>
To achieve this goal we started working with EFE, a ethylene forming enzyme from <i>Pseudomas Syringae</i> pv. phaseolicola (<a href="http://parts.igem.org/Part:BBa_K1065002">BBa_K1065002</a>), which were inserted into pSBBs0K-Pspac (IPGT inducible) and  pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for <i>B. subtilis</i> by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
To achieve this goal we started working with EFE, a ethylene forming enzyme from <i>Pseudomas Syringae</i> pv. phaseolicola (<a href="http://parts.igem.org/Part:BBa_K1065002">BBa_K1065002</a>), which were inserted into pSBBs0K-Pspac (IPGT inducible) and  pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for <i>B. subtilis</i> by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
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<img src="https://static.igem.org/mediawiki/2013/8/85/Tn-2013-project_ethylene-BBa_K1065001.jpg">
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<img src="https://static.igem.org/mediawiki/2013/8/85/Tn-2013-project_ethylene-BBa_K1065001.jpg"/>
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Revision as of 08:38, 30 September 2013

Bacillus Subtilis When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:
  1. Bacillus subtilis sporulates and it can be stored in a inactive state;
  2. Bacillus subtilis is not pathogenic;
  3. we have always worked only with E. coli and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!
Bacillus subtilis would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a B. fruity home edition that exploits this principle.
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).
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