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Team:DTU-Denmark/Notebook/19 September 2013
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| - | This experiment is based on [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Experiment 2]] for the Nir transformant, with some changes. | + | This experiment is based on [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Protocol of Experiment 2]] for the Nir transformant, with some changes. |
The experiment started with a temperature of around 37 <sub>o</sub> C, initial OD of 0.1036 and initial volume of 200 ml. | The experiment started with a temperature of around 37 <sub>o</sub> C, initial OD of 0.1036 and initial volume of 200 ml. | ||
After resuspension the OD was really low around 0.0329. | After resuspension the OD was really low around 0.0329. | ||
| - | The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample. | + | The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample. |
==Results== | ==Results== | ||
Revision as of 09:57, 1 October 2013
19 September 2013
Contents |
Lab 115
Main purpose
The purpose of this experiment is to measure nitric and nitrous oxide from the Nir transformant anaerobically while we spike it with nitrite.
The Experiment 2 describes further our investigation.
Who was in the lab
Kasia, Ariadni
Procedure
This experiment is based on Protocol of Experiment 2 for the Nir transformant, with some changes.
The experiment started with a temperature of around 37 o C, initial OD of 0.1036 and initial volume of 200 ml. After resuspension the OD was really low around 0.0329.
The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample.
Results
Colorimetric results
Ranges
- Measuring range 5-150 mg/L NH4-N
- Measuring range 0.02-1 mg/L NO2-N
| salt (mg/L) | sample 1 | sample 2 | sample after spike | sample 4 | sample 5 |
|---|---|---|---|---|---|
| ammonium | <2 | <2 | <2 | <2 | <2 |
| nitrite | <0.02 | <0.02 | 0.74 | 1.24 | 1.34 |
Nir Transformed E. coli
Conclusion
The NO sensor was drifting and no proper results were obtained.
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