Exeter/10 July 2013

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{{:Team:Exeter/Template/Header}}
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== Morning ==
== Morning ==
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- BBa_K322122 (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.
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- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.
MiniPrep of the following Parts:
MiniPrep of the following Parts:
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- BBa_K322122, cyan pigment
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- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment
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- BBa_K592012, magenta pigment
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- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
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- BBa_K592010, yellow pigment
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- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
Plasmids extracted are now in storage at -20&deg;C.
Plasmids extracted are now in storage at -20&deg;C.
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'''Liquid cultures of the blue light module and magenta pigment'''
'''Liquid cultures of the blue light module and magenta pigment'''
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- BBa_K592010, yellow pigment
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- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
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- BBa_K592012, magenta pigment
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- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
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- BBa_B0015, a terminator
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- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator
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- BBa_K592005, FixJ protein intermediate
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- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate
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- BBa_K592004, blue light sensor
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- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor
'''Retry of BBa_B0034 transformation'''
'''Retry of BBa_B0034 transformation'''
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Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
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==(Next day, results of BBa_B0034 transformation)==
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==(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)==
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We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.
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{{:Team:Exeter/Template/Footer}}

Latest revision as of 19:53, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Morning

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.

MiniPrep of the following Parts:

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

Plasmids extracted are now in storage at -20°C.

Afternoon

Liquid cultures of the blue light module and magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator

- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate

- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)

We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/10_July_2013"