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Team:UNITN-Trento/Parts
From 2013.igem.org
(Difference between revisions)
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065302">BBa_K1065302</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light circuit to produce amilGFP</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | A device to characterize our improved part BBa_K1065305 controlled by pLac promoter. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065305">BBa_K1065305</a></td> |
| - | <td class="name"> | + | <td class="name">BBa_K952003 improved</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065309">BBa_K1065309</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light ethylene producing device</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> |
| - | <td class="name"> | + | <td class="name">Complete blue-light sensing circuit with an inverter</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/1/ | + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> |
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065311">BBa_K1065311</a></td> |
| - | <td class="name"> | + | <td class="name">Complete blue-light sensing device with inverter, amilCP and EFE</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065306">BBa_K1065306</a></td> |
| - | <td class="name"> | + | <td class="name">J23100+Yf1+FixJ</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). | |
</td> | </td> | ||
</tr> | </tr> | ||
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065307">BBa_K1065307</a></td> |
| - | <td class="name"> | + | <td class="name">J23100+Yf1+FixJ (with terminators)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | |
| - | + | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065308">BBa_K1065308</a></td> |
| - | <td class="name"> | + | <td class="name">J23100+Yf1+FixJ (with terminators)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/Tn-2013-parts-Banana-parts.png" /> | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065303">BBa_K1065303</a></td> |
| - | <td class="name"> | + | <td class="name">Yf1+FixJ+pFixK2</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | Yf1 and FixJ | + | The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. |
</td> | </td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td rowspan="2" class="grade"> | <td rowspan="2" class="grade"> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2013/1/1e/Tn-2013-parts-Banana-green-parts.png" /> | |
| - | + | ||
</td> | </td> | ||
| - | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065300">BBa_K1065300</a></td> |
| - | <td class="name"> | + | <td class="name">Blue light circuit to produce amilGFP</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="descr"> | <td colspan="2" class="descr"> | ||
| - | + | It does not work beacuse it lacks the RBS before the amilGFP gene. | |
</td> | </td> | ||
</tr> | </tr> | ||
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<div class="container"> | <div class="container"> | ||
| - | + | <div class="sheet-2"> | |
| - | + | <a href="https://2013.igem.org/Team:UNITN-Trento/Modeling"> | |
| - | + | <img class="tn-arr-prev" src="https://static.igem.org/mediawiki/2013/b/be/Tn-2013-arr-Parts_prev.png" /> | |
| - | + | </a> | |
| - | + | <a href="javascript:toTop('#tn-main-wrap-wrap');"> | |
| - | + | <img src="https://static.igem.org/mediawiki/2013/6/6e/Tn-2013-arr-AAA_TOP.png" /> | |
| - | + | </a> | |
| - | + | <a href="https://2013.igem.org/Team:UNITN-Trento/Project/Datapage"> | |
| - | + | <img class="tn-arr-next" src="https://static.igem.org/mediawiki/2013/f/fb/Tn-2013-arr-Parts_next.png" /> | |
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<!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/e/ef/Tn-2013-headerbg-Sfondogr2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/5/52/Tn-2013-headerbg-Sfondogr2_OR.jpg</html>}} | <!--end content--></html>|<html>https://static.igem.org/mediawiki/2013/e/ef/Tn-2013-headerbg-Sfondogr2.jpg</html>|<html>https://static.igem.org/mediawiki/2013/5/52/Tn-2013-headerbg-Sfondogr2_OR.jpg</html>}} | ||
Revision as of 14:59, 3 October 2013
In this beautiful page you can find the fruits of our summer’s efforts, otherwise you can just go to the official page.
The number of bananas quantifies how much we like each part:
: This is an intermediate part and it has not been characterized.
: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed. - : the part was characterized but doesn't work always as espected.
- : the part works as expected and has been characterized.
♫ ♪ “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” ♪ ♫
[cit. The Banana Splits – The Tra La La Song]
|
|
BBa_K1065000 | EFE |
| Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. | ||
|
|
BBa_K1065002 | EFE with double-terminator |
| EFE followed by the double terminator BBa_B0015. | ||
|
|
BBa_K1065001 | EFE arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
|
|
BBa_K1065100 | BSMT1 |
| This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3. | ||
|
|
BBa_K1065103 | PchBA |
| PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. | ||
|
|
BBa_K1065104 | SAM synthetase |
| This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules. | ||
|
|
BBa_K1065101 | BSMT1 arabinose-regulated |
| A methyltransferase (BSMT1) regulated by the araCpBAD promoter. | ||
|
|
BBa_K1065105 | SAM synthetase under pLac promoter |
| This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter. | ||
|
|
BBa_K1065102 | PchBA under pLac and BSMT1 arabinose-regulated |
| The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter. | ||
|
|
BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production. | ||
|
|
BBa_K1065302 | Blue light circuit to produce amilGFP |
| A device to characterize our improved part BBa_K1065305 controlled by pLac promoter. | ||
|
|
BBa_K1065305 | BBa_K952003 improved |
| No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works. | ||
|
|
BBa_K1065309 | Blue light ethylene producing device |
| Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. | ||
|
|
BBa_K1065310 | Complete blue-light sensing circuit with an inverter |
| Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | ||
|
|
BBa_K1065311 | Complete blue-light sensing device with inverter, amilCP and EFE |
| Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. | ||
|
|
BBa_K1065306 | J23100+Yf1+FixJ |
| Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). | ||
|
|
BBa_K1065307 | J23100+Yf1+FixJ (with terminators) |
| The same as BBa_K1065306, just with the terminators at the end. This part was not submitted to the registry. If you are interested please contact us. | ||
|
|
BBa_K1065308 | J23100+Yf1+FixJ (with terminators) |
| This is an intermediate part to build a blue light inducible genetic circuit with the inverter. This part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact us. | ||
|
|
BBa_K1065303 | Yf1+FixJ+pFixK2 |
| The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. | ||
|
|
BBa_K1065300 | Blue light circuit to produce amilGFP |
| It does not work beacuse it lacks the RBS before the amilGFP gene. | ||
|
|
BBa_K1065201 | pXyl+GFP |
| A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | ||
|
|
BBa_K1065203 | Efe+Bba_B0015 in pSpac. |
| EFE in pSpac vector, specific for B. subtilis. | ||
|
|
BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
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