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Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 19th July.html
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Latest revision as of 20:06, 3 October 2013
Trojan Horse
ASDF19th July
- Conjugation results :
- Controls => no colonies
- Mix = tapis
To be sure to start only with one clone => restreak some colonies on tet/spec plates
- Transformation by electroporation : goal = get a phage factory.
- We do it three different ways in order to see which one works (best).
- Transform strain containing helper plasmid with phagemid
- Transform strain containing helper plasmid with phagemid
- Co Transform strain with both plasmids at the same time
- From strain sT004, sT005, sT006
- Transform sT004 with pT006
- Transform sT005 with pT005
- Co Transform sT006 with pT005 and pT006
- sM13KO7 (control) : 5,60
- sM13KO7 + pLitmus : 5,70
- sM13KO7 + pLitmus : 5,70
- sLITMUS (control) : 5,60
- sLITMUS + pM13K07 : 5,60
- sLITMUS + pM13K07 : 5,60
- MG16165 : control = 5.70
- MG16165 + p (M13K07) and p(LITMUS) = 5.60
- MG16165 + p (M13K07) and p(LITMUS)= 5.60
Tubes :
Recovery 2h
Plate everything on Kan/ Amp
