Team:UGent/Attributions
From 2013.igem.org
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Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey. | Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey. | ||
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- | Inbio (the UGent centre of expertise for industrial biotechnology and biocatalysis) let us make use of their lab and made sure all the necessary lab equipment was at our disposal. | + | Inbio (the UGent centre of expertise for industrial biotechnology and biocatalysis) let us make use of their lab and made sure all the necessary lab equipment was at our disposal. The whole Inbio staff was always ready to assist and advise us when we asked for help. |
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Inbio provided us with the strain bearing plasmids that contain the <i>ccdA-gfp</i> construct we used (<i>E. coli</i> DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (<i>E. coli</i> MG1655 ΔendA DE3), strains containing plasmids with the T7-ccdB fragment (<i>E. coli</i> DH5a + pXSpFRT-T7ccdB) and the strain we used for making P1 phage lysate (<i>E. coli</i> BW26547), along with a small dose of P1 lysate. Inbio also constructed back-up strains (<i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT & <i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP) in which CIChE could be performed if other experiments failed. | Inbio provided us with the strain bearing plasmids that contain the <i>ccdA-gfp</i> construct we used (<i>E. coli</i> DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (<i>E. coli</i> MG1655 ΔendA DE3), strains containing plasmids with the T7-ccdB fragment (<i>E. coli</i> DH5a + pXSpFRT-T7ccdB) and the strain we used for making P1 phage lysate (<i>E. coli</i> BW26547), along with a small dose of P1 lysate. Inbio also constructed back-up strains (<i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT & <i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP) in which CIChE could be performed if other experiments failed. |
Revision as of 00:24, 4 October 2013
Literature study
Prof. Marjan De Mey and Charlotte Verniers assisted us during brainstorming and advised us during our literature study and the writing and presentation of our paper. Frederik De Bruyn also threw in some ideas during brainstorming.
In the lab
Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey.
Other
The following was also accomplished by the students:
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