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Team:UGent/Parts
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<h2>lacIq-T7-ccdB</h2> | <h2>lacIq-T7-ccdB</h2> | ||
| - | <p>This part contains a <i>ccdB</i> gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit GyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB.</p> | + | <p>This part contains a <i>ccdB</i> gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit GyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB.The inducible promoter is a combination of an operator LacO and a T7-promoter. The operator may be switched on or off. This, in turn, controls the transcription of the gene. The repressors (LacI and LacIq), inhibit the transcription by binding with a regulatory element, called the operator LacO. LacIq binds the operator LacO more tightly than LacI.</p> |
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<center><img src="https://static.igem.org/mediawiki/2013/d/d4/UGent_2013_LaqIq-T7-ccdB.png" width="800"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/d/d4/UGent_2013_LaqIq-T7-ccdB.png" width="800"/></center> | ||
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{{:Team:UGent/Templates/Footer}} | {{:Team:UGent/Templates/Footer}} | ||
{{:Team:UGent/Templates/BaseSponsor}} | {{:Team:UGent/Templates/BaseSponsor}} | ||
Revision as of 18:45, 4 October 2013
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New part<groupparts>iGEM013 UGent</groupparts>lacIq-T7-ccdBThis part contains a ccdB gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit GyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB.The inducible promoter is a combination of an operator LacO and a T7-promoter. The operator may be switched on or off. This, in turn, controls the transcription of the gene. The repressors (LacI and LacIq), inhibit the transcription by binding with a regulatory element, called the operator LacO. LacIq binds the operator LacO more tightly than LacI. 
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