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Team:UGent/Parts
From 2013.igem.org
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<center><img src="https://static.igem.org/mediawiki/2013/d/d4/UGent_2013_LaqIq-T7-ccdB.png" width="800"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/d/d4/UGent_2013_LaqIq-T7-ccdB.png" width="800"/></center> | ||
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| + | <h3>Source</h3> | ||
| + | <p>This part (BBa_K1105000) comes from p10-LacIq-T7<i>ccdB</i>, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+.</p> | ||
Revision as of 18:59, 4 October 2013
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New part
lacIq-T7-ccdBThis part contains a ccdB gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit GyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB.The inducible promoter is a combination of an operator LacO and a T7-promoter. The operator may be switched on or off. This, in turn, controls the transcription of the gene. The repressors (LacI and LacIq), inhibit the transcription by binding with a regulatory element, called the operator LacO. LacIq binds the operator LacO more tightly than LacI.  SourceThis part (BBa_K1105000) comes from p10-LacIq-T7ccdB, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+.
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