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<font size = "4"> Cell Surface Display </font> <BR> | <font size = "4"> Cell Surface Display </font> <BR> | ||
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''Moving on'' | ''Moving on'' | ||
| - | <br>Miniprep of cells transformed with ''INP_Strep-Dead'' gibson product. | + | <br>-Miniprep of cells transformed with ''INP_Strep-Dead'' gibson product. |
| - | <br> DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product. | + | <br> -DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product. |
| - | <br> Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product. | + | <br> -Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product. |
| - | <br> Plating of Streptavdin alive received from Oxford University. | + | <br> -Plating of Streptavdin alive received from Oxford University. |
| - | <font size = "4"> Sensing </font> <BR> | + | <font size = "4"> Sensing-Effector</font> <BR> |
| - | + | ''Making a glycerol Stock'' <BR> | |
| - | We made a 50% glycerol stock of the Bacteria that we | + | -We made a 50% glycerol stock of the Bacteria that we inoculated the day before. |
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Cell Surface Display
Moving on
-Miniprep of cells transformed with INP_Strep-Dead gibson product.
-DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
-Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
-Plating of Streptavdin alive received from Oxford University.
Sensing-Effector
Making a glycerol Stock
-We made a 50% glycerol stock of the Bacteria that we inoculated the day before.
"
