"
Team:UGent/Parts
From 2013.igem.org
| Line 13: | Line 13: | ||
<br><br> | <br><br> | ||
<h3>Source</h3> | <h3>Source</h3> | ||
| - | <p>This part (BBa_K1105000) comes from p10-LacIq-T7<i>ccdB</i>, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+.</p> | + | <p>This part (<A HREF="http://parts.igem.org/Part:BBa_K1105000" target="_blank">BBa_K1105000</A>) comes from p10-LacIq-T7<i>ccdB</i>, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+.</p> |
Revision as of 21:46, 4 October 2013
 |
|
|
New part
lacIq-T7-ccdBThis part contains a ccdB gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit GyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB.The inducible promoter is a combination of an operator LacO and a T7-promoter. The operator may be switched on or off. This, in turn, controls the transcription of the gene. The repressors (LacI and LacIq), inhibit the transcription by binding with a regulatory element, called the operator LacO. LacIq binds the operator LacO more tightly than LacI.  SourceThis part (BBa_K1105000) comes from p10-LacIq-T7ccdB, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+.
|
Tweets van @iGEM_UGent |
|
|
