Team:UGent/Attributions
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Inbio provided us with the strain bearing plasmids that contain the <i>ccdA-gfp</i> construct we used (<i>E. coli</i> DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (<i>E. coli</i> MG1655 ΔendA DE3), strains containing plasmids with the T7-<i>ccdB</i> fragment (<i>E. coli</i> DH5a + pXSpFRT-T7<i>ccdB</i>) and the strain we used for making P1 phage lysate (<i>E. coli</i> BW26547), along with a small dose of P1 lysate. Inbio also constructed back-up strains (<i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT & <i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP) in which CIChE could be performed if other experiments failed. | Inbio provided us with the strain bearing plasmids that contain the <i>ccdA-gfp</i> construct we used (<i>E. coli</i> DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (<i>E. coli</i> MG1655 ΔendA DE3), strains containing plasmids with the T7-<i>ccdB</i> fragment (<i>E. coli</i> DH5a + pXSpFRT-T7<i>ccdB</i>) and the strain we used for making P1 phage lysate (<i>E. coli</i> BW26547), along with a small dose of P1 lysate. Inbio also constructed back-up strains (<i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT & <i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP) in which CIChE could be performed if other experiments failed. | ||
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- | Primers starting with MDM and CGL were designed by Prof. Marjan De Mey. Primers beginning with oMEMO were designed by Inbio. Primers starting with iGEM were designed by students of the UGent iGEM team. Pieter Coussement also designed some primers for a reverse mutation. | + | Primers starting with MDM and CGL were designed by Prof. Marjan De Mey. Primers beginning with oMEMO were designed by Inbio. Primers starting with iGEM were designed by students of the UGent iGEM team. Pieter Coussement also designed some primers for a reverse mutation and provided us with the protocol for this back mutation. |
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All the labwork was conducted by the students of the UGent team. Labwork over the two months was distributed between all students. All results were analysed by the students, especially Jules and Tine. We did however receive invaluable insights from our instructors: Prof. Marjan De Mey, Pieter Coussement, Frederik De Bruyn and Gert Peters.</p> | All the labwork was conducted by the students of the UGent team. Labwork over the two months was distributed between all students. All results were analysed by the students, especially Jules and Tine. We did however receive invaluable insights from our instructors: Prof. Marjan De Mey, Pieter Coussement, Frederik De Bruyn and Gert Peters.</p> |
Revision as of 22:22, 4 October 2013
Literature study
Prof. Marjan De Mey and Charlotte Verniers assisted us during brainstorming and advised us during our literature study and the writing and presentation of our paper. Frederik De Bruyn also threw in some ideas during brainstorming.
In the lab
Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey.
Other
The following was also accomplished by the students:
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