Team:Paris Bettencourt/Results
From 2013.igem.org
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+ | <h2>Background</h2> | ||
+ | <p>CRISPR/Cas systems generate site-specific double strand breaks and have recently be used for genome editing. </p> | ||
+ | </div> | ||
+ | <div class="results"> | ||
+ | <h2>Results</h2> | ||
+ | <ul> | ||
+ | <li>Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks. </li> | ||
+ | <li>Testing the new assembly standard for our cloning.</li> | ||
+ | </ul> | ||
+ | <p></p> | ||
+ | </div> | ||
+ | <div class="biocriks"> | ||
+ | <h2>BioBricks</h2> | ||
+ | <ol> | ||
+ | <li><a href="" target="_blank">BBa_K1137012 (gRNA anti-KAN)</a></li> | ||
+ | <li><a href="" target="_blank">BBa_K1137013 (crRNA anti-KAN)</a></li> | ||
+ | <li><a href="" target="_blank">BBa_K1137014 (tracrRNA-Cas9)</a></li> | ||
+ | <li><a href="" target="_blank">BBa_K1137015 (pRecA-LacZ)</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div style="clear: both;"></div> | ||
+ | <div class="aims"> | ||
+ | <h2>Aims</h2> | ||
+ | <p>Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.</p> | ||
+ | </div> | ||
+ | </div> | ||
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Revision as of 23:37, 4 October 2013
Background
CRISPR/Cas systems generate site-specific double strand breaks and have recently be used for genome editing.
Results
- Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks.
- Testing the new assembly standard for our cloning.
BioBricks
Aims
Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.