Team:Paris Bettencourt/Results

From 2013.igem.org

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      <h2>Background</h2>
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      <p>CRISPR/Cas systems generate site-specific double strand breaks and have recently be used for genome editing.  </p>
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      <h2>Results</h2>
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        <li>Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks. </li>
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        <li>Testing the new assembly standard for our cloning.</li>
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      <h2>BioBricks</h2>
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        <li><a href="" target="_blank">BBa_K1137012 (gRNA anti-KAN)</a></li>
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        <li><a href="" target="_blank">BBa_K1137013 (crRNA anti-KAN)</a></li>
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        <li><a href="" target="_blank">BBa_K1137014 (tracrRNA-Cas9)</a></li>
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        <li><a href="" target="_blank">BBa_K1137015 (pRecA-LacZ)</a></li>
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      </ul>
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      <h2>Aims</h2>
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      <p>Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.</p>
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Revision as of 23:37, 4 October 2013

Background

CRISPR/Cas systems generate site-specific double strand breaks and have recently be used for genome editing.

Results

  • Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks.
  • Testing the new assembly standard for our cloning.

Aims

Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
team2013@igem-paris.org
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