Team:Edinburgh/Project

From 2013.igem.org

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    <li class="side_menu_item" > [[Team:Edinburgh/Project|Overview]] </li>
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    <li class="side_menu_item" >  [[Project_description|Description]]
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              <li class="side_menu_subitem"> [[Metal_promoters|Metal promoters]] </li>
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              <li class="side_menu_subitem"> [[Metal_binding|Metal binding]] </li>
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              <li class="side_menu_subitem"> [[Bioethanol|Bioethanol]] </li>
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              <li class="side_menu_subitem"> [[Aggregation|Aggregation]] </li>
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    <li class="side_menu_item" >  [[Team:Edinburgh/Notebook|Notebook]]
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    <li class="side_menu_item" >  [[Team:Edinburgh/Safety|Safety]]
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    <li class="side_menu_item" >  [[Team:Edinburgh/Parts|Parts submitted]] </li>
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    <li class="side_menu_item" >  [[Results|RESULTS]] </li>
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    <li class="side_menu_item" >  [[Team:Edinburgh/Attributions|Attributions]] </li>
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<h3> Results summary </h3>
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* '''We tested [[Team:Edinburgh/Project/Results/Chassis |the growth]] of our experimental strain, <i>Bacillus subtilis 168</i>, under various conditions relevant to the work we are going to perform.'''
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* '''We tested the Kanamycin resistance conferred by [[Team:Edinburgh/Project/Results/Chassis#vector | pTG262]] in <i>B.subtilis</i> and <i>E.coli</i>.
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* '''We created an assembly to demonstrate that [[Team:Edinburgh/Project/Results/Metal promoters Results | Fur box]] is able to repress gene expression upon exposure to high iron concentrations.'''
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* '''We cloned out and characterised a [[Team:Edinburgh/Project/Results/Metal promoters Results#Fur | Fur transcription factor]].'''
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* '''We cloned a Neisserial Ferric binding protein A ([[Team:Edinburgh/Project/Results/Metal_binding_Results|FbpA]]) as a BioBrick.'''
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* '''We generated a [[Team:Edinburgh/Project/Results/Bioethanol_Results| fusion]] of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''
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* '''We cloned [[Team:Edinburgh/Project/Results/Aggregation Results|SinR]] and analysed biofilm formation in WT <i>B.subtilis</i>.'''
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== '''Currently working on'' ==
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* '''We had a great summer, and plan to have fun at the Regional Jamboree.'''
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* PDC-AdhB Fusion
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* NRPS cloning
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* Fur protein and Fur box characterisation
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* Bacillus NCIB3610 competence and biofilm characterisation
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Latest revision as of 23:57, 4 October 2013

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Results summary

  • We tested the growth of our experimental strain, Bacillus subtilis 168, under various conditions relevant to the work we are going to perform.


  • We tested the Kanamycin resistance conferred by pTG262 in B.subtilis and E.coli.


  • We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.



  • We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.


  • We generated a fusion of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.


  • We cloned SinR and analysed biofilm formation in WT B.subtilis.


  • We had a great summer, and plan to have fun at the Regional Jamboree.

Sulsa.jpg Rsc.jpg Sgm.jpg Sfam.jpg Genabler-logo.png Msd.png
This iGEM team has been funded by the MSD Scottish Life Sciences Fund. The opinions expressed by this iGEM team are those of the team members and do not necessarily represent those of MSD
Retrieved from "http://2013.igem.org/Team:Edinburgh/Project"