Team:TU Darmstadt/materials/Primers
From 2013.igem.org
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+ | VR: ATTACCGCCTTTGAGTGAGC<br> | ||
+ | VF2: TGCCACCTGACGTCTAAGAA<br> | ||
+ | <br> | ||
+ | |||
+ | Suffix-R: TACTAGTAGCGGCCGCTGCAGGGTAA<br> | ||
+ | Prefix-R: GTAGTAGAATTCGCGGCCGCTTCTAG<br> | ||
+ | <br> | ||
+ | |||
+ | LO-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATGATTTGTACAGCTCGTCCATGCC<br> | ||
+ | LO-pre-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTGAGCAAAGGCGAAGAAAATAACATGG<br> | ||
+ | <br> | ||
+ | |||
+ | mKate-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATTAATTCAGTTTATGACCCAGGTTGCTCG<br> | ||
+ | mKate-pre-ATG-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTAAGCAAAGGTGAAGAGTTAATCAAGGAGA<br> | ||
+ | <br> | ||
+ | |||
+ | lssmorange ppr: CCCCCTGCAGGTTTATTATTTGTACAGCTCGTCC<br> | ||
+ | lssmorange pprf: ATATGGCTAGCATGGTGAGCAAAGGCG<br> | ||
+ | <br> | ||
+ | |||
+ | mkate ppr: GTCCCCCTGCAGGTTTATTAATTCAGTTTATG<br> | ||
+ | mkate pprf: TACATATGGCTAGCATGGTAAGCAAAGGTG<br> | ||
+ | <br> | ||
+ | |||
+ | frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG<br> | ||
+ | frag1_for: CGGCCGCTTCTAGAGACATTGATTATTTGCACGGCGT<br> | ||
+ | <br> | ||
+ | |||
+ | frag2_rev: CAGTGTGATTATTAATTCAGTTTATGACCCAGGTTGCTCG<br> | ||
+ | frag2_for: GGCTAGCGGAAAGAGGGGACAAATGTCAAAAATCC<br> | ||
+ | <br> | ||
+ | |||
+ | frag3_rev: ATCAATGTAAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCT<br> | ||
+ | frag3_for: TTAATAATCACACTGGCTCACCTTCGG<br> | ||
+ | <br> | ||
+ | |||
+ | frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG<br> | ||
+ | frag4_for: ATTATTTACATTGATTATTTGCACGGCGT<br> | ||
+ | <br> | ||
+ | |||
+ | frag5_rev: CGGCCGCTACTAGTATTATCATTTGTACAGCTCGTCCATGC<br> | ||
+ | frag5_for: TTTTTTTGGGCTAGCGGAAAGAGGGGACAAATGATTAACCG<br> | ||
+ | <br> | ||
+ | |||
+ | vector_rev: CTCTAGAAGCGGCCGCGA<br> | ||
+ | vector_for: TACTAGTAGCGGCCGCTGC<br> | ||
+ | <br> | ||
+ | |||
+ | Pre-PezT: GGATCCTGCAGCGGCCGCTACTAGTACTCGAGCGCTGCTGCCA<br> | ||
+ | For-PezT: ATACCGAATTCGCGGCCGCTTCTAGATGGAAATCCAAGATTATACTG<br> | ||
+ | <br> | ||
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Latest revision as of 01:43, 5 October 2013
Primers
VR: ATTACCGCCTTTGAGTGAGC
VF2: TGCCACCTGACGTCTAAGAA
Suffix-R: TACTAGTAGCGGCCGCTGCAGGGTAA
Prefix-R: GTAGTAGAATTCGCGGCCGCTTCTAG
LO-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATGATTTGTACAGCTCGTCCATGCC
LO-pre-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTGAGCAAAGGCGAAGAAAATAACATGG
mKate-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATTAATTCAGTTTATGACCCAGGTTGCTCG
mKate-pre-ATG-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTAAGCAAAGGTGAAGAGTTAATCAAGGAGA
lssmorange ppr: CCCCCTGCAGGTTTATTATTTGTACAGCTCGTCC
lssmorange pprf: ATATGGCTAGCATGGTGAGCAAAGGCG
mkate ppr: GTCCCCCTGCAGGTTTATTAATTCAGTTTATG
mkate pprf: TACATATGGCTAGCATGGTAAGCAAAGGTG
frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag1_for: CGGCCGCTTCTAGAGACATTGATTATTTGCACGGCGT
frag2_rev: CAGTGTGATTATTAATTCAGTTTATGACCCAGGTTGCTCG
frag2_for: GGCTAGCGGAAAGAGGGGACAAATGTCAAAAATCC
frag3_rev: ATCAATGTAAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCT
frag3_for: TTAATAATCACACTGGCTCACCTTCGG
frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag4_for: ATTATTTACATTGATTATTTGCACGGCGT
frag5_rev: CGGCCGCTACTAGTATTATCATTTGTACAGCTCGTCCATGC
frag5_for: TTTTTTTGGGCTAGCGGAAAGAGGGGACAAATGATTAACCG
vector_rev: CTCTAGAAGCGGCCGCGA
vector_for: TACTAGTAGCGGCCGCTGC
Pre-PezT: GGATCCTGCAGCGGCCGCTACTAGTACTCGAGCGCTGCTGCCA
For-PezT: ATACCGAATTCGCGGCCGCTTCTAGATGGAAATCCAAGATTATACTG