Team:TU Darmstadt/materials/Buffers

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<font size="8" color="#F0F8FF" face="Arial regular">Labbook |</font>
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<h2><font size="6" color="#F0F8FF" face="Arial regular">Buffers</font></h2>
<h2><font size="6" color="#F0F8FF" face="Arial regular">Buffers</font></h2>
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Tris
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Tris (1M, pH 7,5)
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<li>Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol).</li>
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<li>60,5g Tris base</li>
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<li>adjust pH to 7,5 using 5M HCL</li>
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<li>add to 500 mL>
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1x PBS
1x PBS
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<li>8.18 g NaCl (140 mM)</li>
<li>8.18 g NaCl (140 mM)</li>
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<li>0.24 g KH<sub>2</sub>PO<sub>4</sub> (1.8 mM)</li>
<li>0.24 g KH<sub>2</sub>PO<sub>4</sub> (1.8 mM)</li>
<li>Add. 1L dH<sub>2</sub>O</li>
<li>Add. 1L dH<sub>2</sub>O</li>
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<li>Adjust to pH 7.4
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<li>Adjust to pH 7.4</li>
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TE buffer
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<li>10 mM Tris, bring to pH 8.0 with HCl</li>
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<li>1 mM EDTA (Ethylenediaminetetraacetic acid)</li>
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50x TAE
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<li> 242g Tris base in water</li>
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<li> add 57.1mL glacial acetic acid</li>
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<li>100mL of 500mM EDTA (pH 8.0) solution</li>
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<li>add up to 1 litre of water</li>
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Latest revision as of 03:28, 5 October 2013







Lab book | Materials | Protocols

Buffers


Tris (1M, pH 7,5)

  • 60,5g Tris base
  • adjust pH to 7,5 using 5M HCL
  • add to 500 mL>

1x PBS

  • 8.18 g NaCl (140 mM)
  • 0.2 g KCl (2.7 mM)
  • 1.77 g Na2HPO4 (10 mM)
  • 0.24 g KH2PO4 (1.8 mM)
  • Add. 1L dH2O
  • Adjust to pH 7.4

TE buffer

  • 10 mM Tris, bring to pH 8.0 with HCl
  • 1 mM EDTA (Ethylenediaminetetraacetic acid)

50x TAE

  • 242g Tris base in water
  • add 57.1mL glacial acetic acid
  • 100mL of 500mM EDTA (pH 8.0) solution
  • add up to 1 litre of water

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