Team:Imperial College/Home2

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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/3/3c/Thumb_Mountains42.png" alt="image01" width="65" height="65" data-large="https://static.igem.org/mediawiki/2013/5/54/Mountains42.png" data-description="Europe's Newest Mountain, Mount Wasted" /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/9/9d/Slide01.jpg" alt="image01" width="65" height="65" data-large="https://static.igem.org/mediawiki/2013/9/9d/Slide01.jpg" data-description="Waste is a resource! We have engineered a system to divert mixed, non-recyclable waste away from landfill sites and incinerators. Our system uses the waste to produce bioplastic which can be 3D- printed and used for medical applications." /></a></li>
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  <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/d/d4/Thumb_Very_stressed_ecoli2.jpg" data-large="https://static.igem.org/mediawiki/2013/2/2f/Very_stressed_ecoli2.jpg" alt="image02" data-description="Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM for 48 hours." /></a></li>
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  <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/4/41/Slide02.jpg" data-large="https://static.igem.org/mediawiki/2013/4/41/Slide02.jpg" alt="image02" data-description="Resource-full waste: the first module in our system." /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/1/15/Thumb_Jar_of_SRF2.jpg" data-large="https://static.igem.org/mediawiki/2013/e/e8/Jar_of_SRF2.jpg" alt="image03" data-description="Solid Recovered Fuel (SRF)" /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/c/cd/Slide03.jpg" data-large="https://static.igem.org/mediawiki/2013/c/cd/Slide03.jpg" alt="image03" data-description="Bioplastic production (left) native phaCAB (BBa_K934001), (Right) Imperial Biobrick hybrid phaCAB (BBa_K1149051)" /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/f/f9/Thumb_Waste_cocktail.png" data-large="https://static.igem.org/mediawiki/2013/2/2e/Waste_cocktail2.png" alt="image04" data-description="(A) WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 7 days growth in SRF. " /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/c/c3/Slide04.jpg" data-large="https://static.igem.org/mediawiki/2013/c/c3/Slide04.jpg" alt="image04" data-description="Plastic Fantastic: the second module in our system" /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/0/08/Thumbs_PBS_Plus_Waste2.jpg" data-large="https://static.igem.org/mediawiki/2013/1/19/PBS_Plus_Waste2.jpg" alt="image05" data-description="(A) SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 6 days growth in SRF." /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/c/c1/Slide05.jpg" data-large="https://static.igem.org/mediawiki/2013/c/c1/Slide05.jpg" alt="image05" data-description="Our system allows for the complete biological recycling of P(3HB)." /></a></li>
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                                     <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/3/3c/Thumb_Mountains42.png" alt="image01" width="65" height="65" data-large="https://static.igem.org/mediawiki/2013/5/54/Mountains42.png" data-description="Europe's Newest Mountain, Mount Wasted" /></a></li>
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                                     <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/5/54/Slide06.jpg" alt="image01" width="65" height="65" data-large="https://static.igem.org/mediawiki/2013/5/54/Slide06.jpg" data-description="Our system is designed to be industrially scaleable. " /></a></li>
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  <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/d/d4/Thumb_Very_stressed_ecoli2.jpg" data-large="https://static.igem.org/mediawiki/2013/2/2f/Very_stressed_ecoli2.jpg" alt="image02" data-description="Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM for 48 hours." /></a></li>
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  <li><a href="#"><img src="https://static.igem.org/mediawiki/2013/b/b5/Slide07.jpg" data-large="https://static.igem.org/mediawiki/2013/b/b5/Slide07.jpg" alt="image02" data-description="The mixed waste we used in our experiments was sourced from the Powerday recycling centre, West London." /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/1/15/Thumb_Jar_of_SRF2.jpg" data-large="https://static.igem.org/mediawiki/2013/e/e8/Jar_of_SRF2.jpg" alt="image03" data-description="Solid Recovered Fuel (SRF)" /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/9/97/Slide08.jpg" data-large="https://static.igem.org/mediawiki/2013/9/97/Slide08.jpg" alt="image03" data-description="We have also started our own art movement, with our highly cultured E. coli" /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/f/f9/Thumb_Waste_cocktail.png" data-large="https://static.igem.org/mediawiki/2013/2/2e/Waste_cocktail2.png" alt="image04" data-description="(A) WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 7 days growth in SRF. " /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/d/dc/Slide09.jpg" data-large="https://static.igem.org/mediawiki/2013/d/dc/Slide09.jpg" alt="image04" data-description="Our recycling posters were developed with advice from Imperial's own waste management team. We posted them around campus" /></a></li>
                                      
                                      
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/0/08/Thumbs_PBS_Plus_Waste2.jpg" data-large="https://static.igem.org/mediawiki/2013/1/19/PBS_Plus_Waste2.jpg" alt="image05" data-description="(A) SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 6 days growth in SRF." /></a></li>
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<li><a href="#"><img src="https://static.igem.org/mediawiki/2013/7/77/Slide10.jpg" data-large="https://static.igem.org/mediawiki/2013/7/77/Slide10.jpg" alt="image05" data-description="Quotes from our human practices" /></a></li>
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<p>Accumulation of waste represents a considerable problem to humanity. <strong>Over  the next 50 years, the global community will produce approximately 2  trillion tonnes of waste, or 2.5 times the weight of Mount Everest.</strong>
 
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  </p>  <p>Traditionally, mixed non-recyclable waste is sent to landfill or  for incineration, both of which result in environmental damage. The  detrimental effects are perpetrated by the plastic degradation into  toxic byproducts and the production of greenhouse gases by these  processes. We propose to upcycle this mixed waste into the bioplastic  poly-3-hydroxybutyrate (P3HB) to create a closed loop recycling system.  Our engineered E. coli will operate within sealed bioreactors. In the  future we picture the use of our system in a variety of contexts as part  of our M.A.P.L.E. (Modular And Plastic Looping E.coli) system. </p>
 
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<div  style="  width:290px; margin-bottom:3px" c> <a href="https://2013.igem.org/Team:Imperial_College/Judging_Criteria"><img src="https://static.igem.org/mediawiki/2013/e/ea/Button_judge.jpg" alt="Judging criteria" width="290"  /></a> </div>
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<a href="https://2013.igem.org/Team:Imperial_College/mainresults"><img src="https://static.igem.org/mediawiki/2013/3/33/Button_data.jpg" alt="Main data" width="290"  /></a>
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    <td valign="top"><h2><a href=" https://2013.igem.org/Team:Imperial_College/Waste_Degradation:_SRF">Resourceful Waste</a></h2>
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      <p>Non-recyclable waste is sourced from a recycling
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        centre, placed in a bioreactor with our M.A.P.L.E
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        system which degrades the waste and synthesises
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    the bioplastic P(3HB). </p></td>
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    <td valign="top"><h2 class="style2"><a href="https://2013.igem.org/Team:Imperial_College/BioPlastic_Recycling:_PHB">Plastic Fantastic</a></h2>
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      <p>Plastic Fantastic is a complete P(3HB) bioplastic
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        recycling platform, where P(3HB) is degraded
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        into monomeric form and then re-polymerised
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    back into de novo P(3HB) for future applications. </p>      <h2>&nbsp;</h2></td>  </tr>
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<a class="twitter-timeline"  href="https://twitter.com/search?q=%23imperialigem"  data-widget-id="393312753730859008">Tweets about "#imperialigem"</a>
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<div style="clear:both;height:1px;">&nbsp;</div>
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Latest revision as of 09:59, 24 October 2013

At a glance
Judging criteria
Main data

Resourceful Waste

Non-recyclable waste is sourced from a recycling centre, placed in a bioreactor with our M.A.P.L.E system which degrades the waste and synthesises the bioplastic P(3HB).

Plastic Fantastic

Plastic Fantastic is a complete P(3HB) bioplastic recycling platform, where P(3HB) is degraded into monomeric form and then re-polymerised back into de novo P(3HB) for future applications.

 
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